The geometric control of E14 and R1 mouse embryonic stem cell pluripotency by plasma polymer surface chemical gradients

Abstract Plasma polymer surfaces were fabricated such that the cell response to a range of carboxylic acid concentrations on a single sample could be investigated. Surface chemical gradients from hydrophobic plasma polymerised octadiene (OD) to a more hydrophilic plasma polymerised acrylic acid (AA)...

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Bibliographic Details
Published in:Biomaterials 2009-02, Vol.30 (6), p.1066-1070
Main Authors: Wells, Nicola, Baxter, Melissa A, Turnbull, Jeremy E, Murray, Patricia, Edgar, David, Parry, Kristina L, Steele, David A, Short, Robert D
Format: Article
Language:English
Subjects:
Acrylates - pharmacology
Adhesiveness - drug effects
Advanced Basic Science
Animals
Cell Adhesion - drug effects
Cell culture
Cell Differentiation - drug effects
Cell Line
Cell spreading
Cell viability
Culture Media
Dentistry
Embryonic Stem Cells - cytology
Embryonic Stem Cells - drug effects
Mice
Plasma polymerisation
Pluripotent Stem Cells - cytology
Pluripotent Stem Cells - drug effects
Polymers - pharmacology
Surface Properties - drug effects
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Summary:Abstract Plasma polymer surfaces were fabricated such that the cell response to a range of carboxylic acid concentrations on a single sample could be investigated. Surface chemical gradients from hydrophobic plasma polymerised octadiene (OD) to a more hydrophilic plasma polymerised acrylic acid (AA) were formed on glass coverslips. Surface characterisation of the chemical gradients was performed using X-ray photoelectron spectroscopy to determine elemental composition. Following culture of E14 and R1 mouse embryonic stem cells (mES) in differing culture media, cell pluripotency was determined by alkaline phosphatase staining. The results demonstrate that for these cell lines the capacity for self-renewal is maintained if the cells are restricted in their spreading to
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2008.10.027