Germ‐line specific variants of components of the mitochondrial outer membrane import machinery in Drosophila

A search of the Drosophila genome for genes encoding components of the mitochondrial translocase of outer membrane (TOM) complex revealed duplication of genes encoding homologues of Tom20 and Tom40. Tom20 and Tom40 were represented by two differentially expressed homologues in the Drosophila genome....

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Bibliographic Details
Published in:FEBS letters 2004-08, Vol.572 (1-3), p.141-146
Main Authors: Hwa, Jennifer J, Zhu, Alan J, Hiller, Mark A, Kon, Charlene Y, Fuller, Margaret T, Santel, Ansgar
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cannonball
Conserved Sequence
Drosophila
Drosophila - genetics
Drosophila Proteins - chemistry
Drosophila Proteins - genetics
Gene duplication
Genetic Variation
Genome
Germ-Line Mutation - genetics
Intracellular Membranes
Membrane Transport Proteins - chemistry
Membrane Transport Proteins - genetics
Mitochondria - genetics
Mitochondrial import
Mitochondrial protein
Mitochondrial Proteins - chemistry
Mitochondrial Proteins - genetics
Molecular Sequence Data
Receptors, Cell Surface - chemistry
Receptors, Cell Surface - genetics
Receptors, Cytoplasmic and Nuclear - chemistry
Receptors, Cytoplasmic and Nuclear - genetics
Sequence Alignment
Sequence Homology, Amino Acid
Spermatogenesis
Testis-specific
TOM complex
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Summary:A search of the Drosophila genome for genes encoding components of the mitochondrial translocase of outer membrane (TOM) complex revealed duplication of genes encoding homologues of Tom20 and Tom40. Tom20 and Tom40 were represented by two differentially expressed homologues in the Drosophila genome. While dtom20 and dtom40 appeared to be expressed ubiquitously, the second variants, called tomboy20 and tomboy40, were expressed only in the male germ‐line. Transcripts for tomboy20 and tomboy40 were detected in primary spermatocytes as well as post‐meiotic stages. Transcription of tomboy20 and tomboy40 in spermatocytes was not dependent on the transcription factor Cannonball, which is responsible for controlling expression of gene products exclusively required for post‐meiotic germ cell differentiation. Epitope‐tagging and transient expression of dTom20 and Tomboy40 in mammalian cell culture showed proper targeting to mitochondria.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2004.07.025