Repair of rabbit segmental defects with the thrombin peptide, TP508

• Published in: Journal of orthopaedic research 2004-09, Vol.22 (5), p.1094-1099
• Main Authors: Sheller, Michael R, Crowther, Roger S, Kinney, John H, Yang, Jinping, Di Jorio, Sherry, Breunig, Tom, Carney, Darrell H, Ryaby, James T
• Format: Article
• Language: English
• Subjects: Animals; Biomechanical Phenomena; Bone Regeneration - drug effects; Male; Mechanical testing; Microspheres; Microtomography; Peptide Fragments - administration & dosage; Peptide Fragments - pharmacology; Rabbit model; Rabbits; Radiography; Radius; Radius - diagnostic imaging; Segmental bone defect; Synchrotron radiation; Thrombin - administration & dosage; Thrombin - pharmacology; Thrombin related peptide; Ulna; Ulna - diagnostic imaging; Wound Healing - drug effects
• ISSN: 0736-0266; 1554-527X
• DOI: 10.1016/j.orthres.2004.03.009

The synthetic peptide, TP508 (Chrysalin ®), was delivered to rabbit segmental bone defects in biodegradable controlled-release PLGA microspheres to determine its potential efficacy for enhancing healing of non-critically and critically sized segmental defects. Non-critically sized radial defects were created in the forelimbs of New Zealand White rabbits, which were randomized into three treatment groups receiving 10, 50 and 100 μg doses of TP508 in the right radius and control microspheres (without TP508) in the left radius. Torsional testing of the radii at six weeks showed a significant increase in ultimate torque, failure torque, ultimate energy, failure energy, and stiffness when treated with TP508 compared to controls ( p