Coating of titanium implants with type-I collagen

Purpose: Type-I collagen, the major structural protein in bone, has beneficial properties regarding bone regeneration. Little is known about the potential effects of collagen coating on orthopedic implants. Methods: 3 to 6 μg/cm 2 of lyophilized type-I collagen was absorbed on titanium rods. Six coa...

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Bibliographic Details
Published in:Journal of orthopaedic research 2004-09, Vol.22 (5), p.1025-1034
Main Authors: Rammelt, Stefan, Schulze, Eva, Bernhardt, Ricardo, Hanisch, Uwe, Scharnweber, Dieter, Worch, Hartmut, Zwipp, Hans, Biewener, Achim
Format: Article
Language:English
Subjects:
Animals
Cathepsin D - analysis
Collagen
Collagen Type I
Immunohistochemistry
Implant coating
Implants, Experimental
Male
Rats
Rats, Wistar
Tibia - surgery
Titanium
Undecalcified bone sections
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Summary:Purpose: Type-I collagen, the major structural protein in bone, has beneficial properties regarding bone regeneration. Little is known about the potential effects of collagen coating on orthopedic implants. Methods: 3 to 6 μg/cm 2 of lyophilized type-I collagen was absorbed on titanium rods. Six coated and uncoated pins of 0.9 mm diameter were inserted into the tibia of adult male Wistar rats for 1, 2, 4, 7, 14, and 28 days. Specimens were embedded in methacrylate-based Technovit 9100N resin. From one portion cutting and grinding sections were obtained. The implant was removed from the other half that was depolymerized, sectioned, and mounted for immunohistochemistry. Results: At day 4, the interface around the collagen-coated implants displayed a granulation tissue with higher numbers of cathepsin D-positive mononucleated cells compared to the uncoated implants ( p
ISSN:0736-0266
1554-527X
DOI:10.1016/j.orthres.2004.02.011