Expression of cytokines and respiratory burst activity of milk cells in response to Azadirachta indica during bovine mastitis

The immuno therapeutic potential of hydro-methanolic extract of Azadirachta indica (A. indica) was studied during bovine clinical mastitis (CM). The somatic cell count (SCC), total bacterial count (TBC), milk differential leukocyte count (DLC), hydrogen peroxide (H₂O₂), superoxide anion (O₂ ⁻) produ...

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Bibliographic Details
Published in:Tropical animal health and production 2009-02, Vol.41 (2), p.189-197
Main Authors: De, U. K, Mukherjee, Reena
Format: Article
Language:English
Subjects:
Animals
Antibiotics
Azadirachta - chemistry
Azadirachta indica
Biomedical and Life Sciences
Cattle
Cell Count - veterinary
Cytokine
Cytokines - immunology
Cytokines - metabolism
Female
Herbs
Hydrogen peroxide
Leukocyte Count - veterinary
Life Sciences
Lymphocytes
mastitis
Mastitis, Bovine - drug therapy
Milk
Milk - cytology
Milk - immunology
Milk - microbiology
Neutrophils - immunology
Neutrophils - metabolism
Original Paper
Phytotherapy - methods
Phytotherapy - veterinary
Plant Extracts - therapeutic use
Respiratory Burst
Time Factors
Veterinary Medicine/Veterinary Science
Zoology
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Summary:The immuno therapeutic potential of hydro-methanolic extract of Azadirachta indica (A. indica) was studied during bovine clinical mastitis (CM). The somatic cell count (SCC), total bacterial count (TBC), milk differential leukocyte count (DLC), hydrogen peroxide (H₂O₂), superoxide anion (O₂ ⁻) production and interleukin- 2 (IL-2) and gamma interferon (IFN-γ) cytokines expression were studied before and after intramammary infusion of A. indica extract in diseased cows. The results revealed that A. indica treatment significantly (P < 0.05) decreased the SCC, TBC, milk neutrophil percent and significantly (P < 0.05) enhanced milk lymphocyte percent, H₂O₂ and O₂ ⁻ production by milk cells. The IL-2 and IFN-γ were expressed in normal healthy cows and diseased cows after A. indica treatment, whereas both the cytokines could not be expressed in cows treated with antibiotic and in untreated diseased cows. The results of the present study indicated anti inflammatory, antibacterial and immunomodulatory potential of the herb, these activities could be due to the presence of bioactive principle in the extract. This is a preliminary trial indicated beneficial effect of the herb against bovine mastitis it can be developed as an alternative therapy where the use of antibiotics is normally restricted.
ISSN:0049-4747
1573-7438
DOI:10.1007/s11250-008-9174-x