Atomic resolution analyses of the binding of xylobiose-derived deoxynojirimycin and isofagomine to xylanase Xyn10A

The atomic resolution structures of xylobiose-derived isofagomine and xylobiose-derived deoxynojirimycin in complex with the xylanase Xyn10A from Streptomyces lividans reveal undistorted (4)C(1) chair conformed sugars and, in the case of the deoxynojirimycin analogue, suggest unusual pK(a) changes o...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2004-08 (16), p.1794-1795
Main Authors: Gloster, Tracey M, Williams, Spencer J, Roberts, Shirley, Tarling, Chris A, Wicki, Jacqueline, Withers, Stephen G, Davies, Gideon J
Format: Article
Language:English
Subjects:
1-Deoxynojirimycin - chemistry
Binding Sites
Catalysis
Crystallography, X-Ray
Disaccharides - chemistry
Enzyme Inhibitors - pharmacology
Hydrogen-Ion Concentration
Imino Pyranoses
Piperidines - chemistry
Spectrophotometry, Atomic - methods
Streptomyces lividans - enzymology
Xylosidases - chemistry
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Summary:The atomic resolution structures of xylobiose-derived isofagomine and xylobiose-derived deoxynojirimycin in complex with the xylanase Xyn10A from Streptomyces lividans reveal undistorted (4)C(1) chair conformed sugars and, in the case of the deoxynojirimycin analogue, suggest unusual pK(a) changes of the enzyme's catalytic machinery upon binding.
ISSN:1359-7345
DOI:10.1039/b405152a