Entamoeba histolytica: Effect on virulence, growth and gene expression in response to monoxenic culture with Escherichia coli 055

• Published in: Experimental parasitology 2009-02, Vol.121 (2), p.167-174
• Main Authors: Mendoza-Macías, Claudia Leticia, Barrios-Ceballos, Minerva Paola, de la Peña, Lydia Patricia Cárdenas, Rangel-Serrano, Angeles, Anaya-Velázquez, Fernando, Mirelman, David, Padilla-Vaca, Felipe
• Format: Article
• Language: English
• Subjects: ACP; ADH; alcohol dehydrogenase; Amoeba–bacteria relationship; Animals; annealing control primer; benzyloxycarbonyl- l-arginyl- l-arginine- p-nitroanilide; Biological and medical sciences; Cell Line; Coculture Techniques; Cysteine Endopeptidases - genetics; Cysteine Endopeptidases - metabolism; cysteine protease; DMEM; DNA Primers - chemistry; DNA, Complementary - biosynthesis; DNA, Protozoan - chemistry; Dulbecco’s Modified Eagle’s medium; Entamoeba histolytica; Entamoeba histolytica - genetics; Entamoeba histolytica - growth & development; Entamoeba histolytica - pathogenicity; Escherichia coli; Escherichia coli - growth & development; Escherichia coli - physiology; Fundamental and applied biological sciences. Psychology; Gal/GalNAc; galactose/ N-acetylgalactosamine; Gene expression; Gene Expression Regulation, Developmental; Life cycle. Host-agent relationship. Pathogenesis; Macroarrays; Madin–Darby canine kidney cell line; MDCK; PBS; phosphate-buffered saline; Protozoa; Reverse Transcriptase Polymerase Chain Reaction; RNA, Protozoan - genetics; RNA, Protozoan - isolation & purification; RT-PCR; Virulence; Z-Arg-Arg-pNA
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1016/j.exppara.2008.10.011

Monoxenic cultivation of pathogenic Entamoeba histolytica trophozoites with Escherichia coli serotype 055 which binds strongly to the Gal/GalNAc amoebic lectin, markedly improved the growth of E. histolytica and produced a significant decrease in cysteine proteinase activity and a lower cytopathic activity on monolayer cells after 3 months of monoxenic culture. However, after long term monoxenic culture (12 months) the proteolytic and cytopathic activities were recovered and the amoebic growth reached the maximum yield. Employing the GeneFishing R technology and DNA macroarrays we detected differentially gene expression related to the amoebic interaction with bacteria. A number of differentially expressed genes encoding metabolic enzymes, ribosomal proteins, virulence factors and proteins related with cytoskeletal and vesicle trafficking were found. These results suggest that E. coli 055 has a nutritional role that strongly supports the amoebic growth, and is also able to modulate some biological activities related with amoebic virulence.