Microdistributions of prospective BNCT-compound CuTCPH in tissue sections with a heavy ion microbeam

• Published in: Applied radiation and isotopes 2004-11, Vol.61 (5), p.771-774
• Main Authors: Stoliar, P, Kreiner, A.J, Debray, M.E, Caraballo, M.E, Valda, A.A, Davidson, J, Davidson, M, Kesque, J.M, Somacal, H, DiPaolo, H, Burlon, A.A, Ozafrán, M.J, Vázquez, M.E, Minsky, D, Heber, E.M, Trivillin, V.A, Schwint, A.E
• Format: Article
• Language: English
• Subjects: Animals; Boron Neutron Capture Therapy - instrumentation; Boron Neutron Capture Therapy - methods; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - radiotherapy; Cheek; Cricetinae; Hamster cheek pouch model; Heavy ion microbeam; Mesocricetus; Metalloporphyrins - pharmacokinetics; Metalloporphyrins - therapeutic use; Microdistributions of BNCT-Compound CuTCPH; Mouth Neoplasms - metabolism; Mouth Neoplasms - radiotherapy; PIXE X-ray spectroscopy; Spectrometry, X-Ray Emission; Tissue Distribution
• ISSN: 0969-8043; 1872-9800
• DOI: 10.1016/j.apradiso.2004.05.062

Microdistributions of the prospective BNCT-compound CuTCPH, a carborane-containing tetraphenylporphyrin with one Cu atom in its molecular structure, have been obtained in tissue sections of different organs of tumor-bearing and normal Syrian hamsters injected with the boron compound by employing a heavy ion microbeam. High resolution X-ray spectroscopy following micro-PIXE (Particle Induced X-ray Emission with micrometer-sized beams) with a focused 16O ion beam was used. Focusing was performed with a heavy-ion scanning high-precision magnetic quadrupole triplet microprobe. Squamous Cell Carcinomas were induced on the right Cheek Pouch of Syrian Hamsters (HCP), sampled, cryo-sectioned and freeze-dried. Two-dimensional maps of elemental concentration were obtained by scanning the beam over the samples. Very non-uniform Cu concentrations were found in all sections.