Differential diagnosis of Taenia asiatica using multiplex PCR

Taenia asiatica and T. saginata are frequently confused tapeworms due to their morphological similarities and sympatric distribution in Asian regions. To resolve this problem, a high-resolution multiplex PCR assay was developed to distinguish T. asiatica infections from infection with other human Ta...

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Bibliographic Details
Published in:Experimental parasitology 2009-02, Vol.121 (2), p.151-156
Main Authors: Jeon, Hyeong-Kyu, Chai, Jong-Yil, Kong, Yoon, Waikagul, Jitra, Insisiengmay, Bounnaloth, Rim, Han-Jong, Eom, Keeseon S.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Diagnosis, Differential
Differential diagnosis
DNA Primers - chemistry
DNA, Helminth - chemistry
DNA, Helminth - isolation & purification
DNA, Mitochondrial - chemistry
Feces - parasitology
Fundamental and applied biological sciences. Psychology
Humans
Invertebrates
Life cycle. Host-agent relationship. Pathogenesis
Multiplex PCR
Nemathelminthia. Plathelmintha
Polymerase Chain Reaction - methods
Protozoa
Sensitivity and Specificity
Species Specificity
T. saginata
T. solium
Taenia
Taenia - classification
Taenia - genetics
Taenia asiatica
Taeniasis - diagnosis
Taeniasis - parasitology
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Summary:Taenia asiatica and T. saginata are frequently confused tapeworms due to their morphological similarities and sympatric distribution in Asian regions. To resolve this problem, a high-resolution multiplex PCR assay was developed to distinguish T. asiatica infections from infection with other human Taenia tapeworms. For molecular characterization, the species specificity of all materials used was confirmed by sequencing of the cox1 gene. Fifty-two samples were analyzed in this study, comprising 20 samples of T. asiatica genomic DNA from China, Korea, and the Philippines; 24 samples of T. saginata from Belgium, Chile, China, Ethiopia, France, Indonesia, Korea, Laos, the Philippines, Poland, Taiwan, Thailand, and Switzerland; and 10 samples of T. solium from Cape Verde, China, Honduras, and Korea. The diagnostic quality of the results obtained using PCR and species-specific primers designed from valine tRNA and NADH genes was equal to that based on the nucleotide sequencing of the cox1 gene. Using oligonucleotide primers Ta4978F, Ts5058F, Tso7421F, and Rev7915, the multiplex PCR assay was useful for the differentially diagnosing T. asiatica, T. saginata, and T. solium based on 706-, 629-, and 474-bp bands.
ISSN:0014-4894
1090-2449
DOI:10.1016/j.exppara.2008.10.014