A conserved 37 kDa outer membrane protein of Edwardsiella tarda is an effective vaccine candidate

• Published in: Vaccine 2004-09, Vol.22 (25), p.3411-3418
• Main Authors: Kawai, Kenji, Liu, Ying, Ohnishi, Kouhei, Oshima, Syun-ichirou
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Antibody Specificity; Applied microbiology; Bacterial Outer Membrane Proteins - immunology; Bacterial Vaccines - immunology; Bacteriology; Biological and medical sciences; Blotting, Western; Conserved Sequence; Edwardsiella tarda; Edwardsiella tarda - immunology; Electrophoresis, Polyacrylamide Gel; Enterobacteriaceae Infections - immunology; Enterobacteriaceae Infections - prevention & control; Enterobacteriaceae Infections - veterinary; Enzyme-Linked Immunosorbent Assay; Fish Diseases - immunology; Fish Diseases - prevention & control; Fishes - immunology; Fundamental and applied biological sciences. Psychology; Goats - immunology; Immunoglobulin G - analysis; Immunoglobulin G - biosynthesis; Immunoglobulin M - analysis; Immunoglobulin M - biosynthesis; Microbiology; Molecular Sequence Data; O Antigens - immunology; O Antigens - isolation & purification; Outer membrane protein (OMP); Paralichthys olivaceus; Pleuronectiformes; Rabbits; Serotyping; Survival Analysis; Vaccine candidate; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2004.02.026

An effective vaccine against Edwardsiella tarda has not been reported, one of main reasons is the variation in its serotypes. This study aimed to develop an effective vaccine against different serotypes of E. tarda. A conserved 37 kDa outer membrane protein (OMP) of E. tarda was obtained by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). It showed comprising several proteins by two-dimensional (2D) PAGE analysis and showed a high similarity to glyceraldehyde-3-phosphate dehydrogenase by N-terminal amino acid sequence analysis. Japanese flounder ( Paralichthys olivaceus) vaccinated with the 37 kDa OMP was significantly protected against the infections by different serotypes of E. tarda. A specific antibody was also detected by using ELISA. This study suggests that the 37 kDa OMP is an effective potent vaccine candidate against different serotypes of E. tarda.