A non‐invasive method for measurement of gastric emptying in mice: effects of altering fat content and CCK A receptor blockade

• Published in: Neurogastroenterology and motility 2004-08, Vol.16 (4), p.421-427
• Main Authors: Whited, K. L., Hornof, W. J., Garcia, T., Bohan, D. C., Larson, R. F., Raybould, H. E.
• Format: Article
• Language: English
• Subjects: Animals; devazepide; Devazepide - pharmacology; Dietary Fats - pharmacology; Gastric Emptying - drug effects; Gastric Emptying - physiology; Male; Mice; Mice, Inbred C57BL; nuclear scintigraphy; Radionuclide Imaging - methods; Receptor, Cholecystokinin A - antagonists & inhibitors; Receptor, Cholecystokinin A - physiology; Reproducibility of Results; triglyceride
• ISSN: 1350-1925; 1365-2982
• DOI: 10.1111/j.1365-2982.2004.00529.x

The ability to make repetitive non‐invasive measurements of gastric emptying of nutritive solids in awake, unstressed mice is highly desirable. The aim of the present study was to develop such a technique using nuclear scintigraphy and diets differing in triglyceride content. Awake mice were accustomed to light restraint and to feeding cooked, egg white (0.00 g fat g−1), whole egg (0.10 g fat g−1), or egg yolk (0.31 g fat g−1). Gastric emptying of each diet was measured by labelling the test meals with Technetium99m Mebrofenin and using a conventional gamma camera equipped with a high resolution, parallel hole collimator. Gastric emptying of cooked whole egg was also determined following administration of either vehicle or CCK A receptor antagonist, devazepide. The half‐emptying time (t1/2) significantly increased with increasing triglyceride content from 14 ± 5 min to 51 ± 6 min and 82 ± 4 min for egg white, whole egg and egg yolk, respectively. Administration of devazepide significantly decreased t1/2 of whole egg to 28 ± 2 min. These results demonstrate the sensitivity and predictability of this technique in mice and importantly, provide an opportunity to alter the macronutrient or caloric content of the meal to determine effects on gastric emptying.