Associations of polymorphisms in the Pit-1 gene with growth and carcass traits in Angus beef cattle

• Published in: Journal of animal science 2004-08, Vol.82 (8), p.2229-2233
• Main Authors: Zhao, Q, Davis, M.E, Hines, H.C
• Format: Article
• Language: English
• Subjects: Angus; animal genetics; animal growth; Animal productions; Animals; beef cattle; beef quality; biochemical polymorphism; Biological and medical sciences; Body Composition - genetics; Bos; carcass characteristics; Cattle - genetics; Cattle - growth & development; DNA-Binding Proteins - genetics; Exons; Female; Food industries; Fundamental and applied biological sciences. Psychology; Genetic Linkage; Genetic Markers; genetic variation; genotype; growth traits; insulin-like growth factor I; Insulin-Like Growth Factor I - analysis; Insulin-Like Growth Factor I - genetics; Introns; Linear Models; Male; Meat and meat product industries; molecular sequence data; Pit-1 gene; Polymorphism, Single-Stranded Conformational; Quantitative Trait, Heritable; Random Allocation; Selection, Genetic; sequence analysis; single-stranded conformational polymorphism; Terrestrial animal productions; Transcription Factor Pit-1; Transcription Factors - genetics; Vertebrates
• ISSN: 0021-8812; 1525-3163
• DOI: 10.2527/2004.8282229x

The Pit-1 gene was studied as a candidate for genetic markers of growth and carcass traits. Angus beef cattle that were divergently selected for high- or low-blood serum IGF-I concentration were used in this study. The single-strand conformation polymorphism method was used to identify polymorphism in the Pit-1 gene including regions from intron 2 to exon 6. Two polymorphisms, Pit1I3H (HinfI) and Pit1I3NL (NlaIII), were detected in intron 3 of the Pit-1 gene. One polymorphism, Pit1I4N (BstNI), was found in intron 4, and a single nucleotide polymorphism, Pit1I5, was found in intron 5. The previously reported polymorphism in exon 6, Pit1E6H (HinfI), was also studied in 416 Angus beef cattle. Associations of the polymorphisms with growth traits, carcass traits, and IGF-I concentration were analyzed using a general linear model procedure. No significant associations were observed between these polymorphisms and growth and carcass traits.