The natural intron sequence of human tyrosine pre-transfer RNA is not a temporal stabilizer for cloverleaf structure

We have developed the hyperprocessing technique to evaluate the stability of the cloverleaf shape of pretransfer RNA (pre-tRNA). Application of this strategy to hyperprocessible human tyrosine pre-tRNA indicated that the natural intron sequence did not contribute to stabilization of the cloverleaf s...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2004-08, Vol.68 (8), p.1782-1785
Main Authors: Ando, T. (Toyohashi Univ. of Technology, Aichi (Japan)), Tanaka, T, Kikuchi, Y
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
ELECTROPHORESIS
Escherichia coli
Fundamental and applied biological sciences. Psychology
GENES
Humans
hyperprocessing
Introns - genetics
MAGNESIUM
MANKIND
Molecular Sequence Data
Nucleic Acid Conformation
NUCLEOTIDE SEQUENCE
ribonuclease P
RIBONUCLEASES
RNA Stability - genetics
RNA, Transfer - genetics
STABILITY
TRANSCRIPTION
TRANSFER RNA
Tyrosine - metabolism
tyrosine transfer RNA (tRNA)
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Summary:We have developed the hyperprocessing technique to evaluate the stability of the cloverleaf shape of pretransfer RNA (pre-tRNA). Application of this strategy to hyperprocessible human tyrosine pre-tRNA indicated that the natural intron sequence did not contribute to stabilization of the cloverleaf shape of this pre-tRNA, while the artificial intron with elongated anticodon-stem completely inhibited hyperprocessing of it Our data suggested that the contemporary intron sequence may be a vestige of the ancient pre-biotic world, but not has been retained as a temporal stabilizer of the pre-tRNA before the base modifications.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.68.1782