Measuring partial body potassium in the legs of patients with spinal cord injury: a new approach

• Published in: Journal of applied physiology (1985) 2009-01, Vol.106 (1), p.268-273
• Main Authors: Wielopolski, L, Ramirez, L. M, Spungen, A. M, Swaby, S, Asselin, P, Bauman, W. A
• Format: Article
• Language: English
• Subjects: Absorptiometry, Photon; Biological and medical sciences; Body Composition; Body Weights and Measures - instrumentation; Body Weights and Measures - methods; Calibration; Case-Control Studies; Chronic Disease; Equipment Design; Fundamental and applied biological sciences. Psychology; Humans; Leg; Measurement; Muscle, Skeletal - chemistry; Muscular Atrophy - etiology; Muscular Atrophy - metabolism; Musculoskeletal system; Paralysis; Paralysis - etiology; Paralysis - metabolism; Phantoms, Imaging; Pilot Projects; Potassium; Potassium - analysis; Potassium Radioisotopes - analysis; Reproducibility of Results; Spectrometry, Gamma; Spinal cord injuries; Spinal Cord Injuries - complications; Spinal Cord Injuries - metabolism
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/japplphysiol.90435.2008

1 Brookhaven National Laboratory, Environmental Science Department, Upton, New York; 2 Department of Veterans Affairs Rehabilitation Research and Development Service, Merit Review Program and the Center of Excellence for the Medical Consequences of Spinal Cord Injury; and Medical and Research Services, Veterans Affairs Medical Center, Bronx, New York; and 3 Departments of Medicine and Rehabilitation Medicine, Mount Sinai Medical School, New York, New York Submitted 21 March 2008 ; accepted in final form 12 November 2008 Patients with acute spinal cord injury (SCI) with paralysis experience rapid and marked muscle atrophy below the level of the lesion. Muscle is lost above the lesion due to enforced bed rest associated with immobilization. Presently, there is no viable method to quantify muscle loss between the time of injury to the initiation of rehabilitation and remobilization. Furthermore, to assess the efficacy of any physical or pharmacological intervention necessitates the ability to accurately determine the impact of these treatments on muscle mass and function. Our results are presented from measurements of regional potassium (K) in the legs of persons with chronic SCI. The intracellular body K, comprising 97% of the total body K, is indicative of the metabolically active cell mass, of which over 50% is located in the skeletal muscle (SM). To assess regional variations in SM mass in the legs, a partial body K (PBK) system designed for this purpose was placed on a potentially mobile cart. The SM mass measured by PBK in an able-bodied control cohort ( n = 17) and in patients with chronic SCI ( n = 21) was 17.6 ± 0.86 and 11.0 ± 0.65 kg, respectively, a difference of 37.5%. However, the difference in the lean tissue mass of the legs obtained by dual-energy absorptiometry (DXA) in the same cohorts was 20.5 ± 0.86 and 15.5 ± 0.88 kg, respectively, or a difference of 24.4%. PBK offers a novel approach to obtain regional K measurements in the legs, thus allowing the potential for early and serial assessment of muscle loss in SCI subjects during the acute and subacute periods following paralysis. The basic characteristics and performance of our PBK system and our calibration procedure are described in this preliminary report. in vivo; skeletal muscle; body composition; paraplegia Address for reprint requests and other correspondence: L. Wielopolski, Brookhaven National Laboratory, Bldg. 490D, Upton, NY 11973 (e-mail: lwielo{at}bnl.gov )