Cytokine and chemokine response of bone cells after dentin challenge in vitro

Objective:  The aim of this study was to characterize the effects of dentin extracts on cytokine, chemokine and nitric oxide (NO) production by primary rat bone cells. Study design:  Osteoblastic bone marrow cultures were exposed to particulate (D‐part), non‐particulate (D‐n‐part) and demineralized...

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Bibliographic Details
Published in:Oral diseases 2004-09, Vol.10 (5), p.258-264
Main Authors: Silva, TA, Lara, VS, Rosa, AL, Cunha, FQ
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - analysis
Animals
bone cells
Bone Marrow Cells - immunology
Cell Division
Cell Survival
Cells, Cultured
chemokines
Chemokines, CXC - analysis
cytokines
Cytokines - analysis
dentin
Dentin - immunology
Dentistry
Humans
Intercellular Signaling Peptides and Proteins - analysis
Interleukin-1 - analysis
Interleukin-10 - analysis
Interleukin-6 - analysis
Male
Nitric Oxide - metabolism
Osteoblasts - immunology
Osteogenesis - physiology
Rats
Rats, Wistar
Tissue Extracts - immunology
Tumor Necrosis Factor-alpha - analysis
Up-Regulation
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Summary:Objective:  The aim of this study was to characterize the effects of dentin extracts on cytokine, chemokine and nitric oxide (NO) production by primary rat bone cells. Study design:  Osteoblastic bone marrow cultures were exposed to particulate (D‐part), non‐particulate (D‐n‐part) and demineralized dentin extracts and evaluated for proliferative activity, cell morphology, alkaline phosphatase activity and bone‐like nodule formation. Cytokine production was assessed by enzyme‐linked immunosorbent assay and NO release by the Griess method. Results:  The dentin extracts did not affect osteoblast numbering. Conversely, they up regulated in a dose‐dependent manner the production by the osteoblasts of the pro‐inflammatory interleukin‐1β (IL‐1β), tumor necrosis factor‐α, IL‐6, cytokine‐induced neutrophil chemoattractant‐1, and of the anti‐inflammatory cytokine, IL‐10. The NO production was stimulated only by D‐n‐part. Conclusion:  These results demonstrate that dentin induces the production of inflammatory cytokines by osteoblasts and suggest that pro‐resorptive pathways might be stimulated when dentin molecules come into contact with bone cells during pathological processes associated with dentin and bone matrix dissolution.
ISSN:1354-523X
1601-0825
DOI:10.1111/j.1601-0825.2004.01011.x