Importance of the cytochrome P450 monooxygenase CYP52 family for the sophorolipid-producing yeast Candida bombicola

Three cytochrome P450 monooxygenases belonging to the CYP52 family were isolated from the genome of the sophorolipid-producing yeast Candida bombicola using degenerate PCR and genomic walking. One gene displayed high identity with the CYP52E members and was classified into this group (CYP52E3), wher...

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Bibliographic Details
Published in:FEMS yeast research 2009-02, Vol.9 (1), p.87-94
Main Authors: Van Bogaert, Inge N.A, Demey, Marjan, Develter, Dirk, Soetaert, Wim, Vandamme, Erick J
Format: Article
Language:English
Subjects:
alkane assimilation
Alkanes
Amino Acid Sequence
Candida - enzymology
Candida - genetics
Candida - metabolism
Candida bombicola
Carbon sources
CYP52
Cytochrome
Cytochrome P-450 Enzyme System - genetics
Cytochrome P-450 Enzyme System - physiology
Cytochrome P450
Cytochrome P450 monooxygenase
DNA, Fungal - genetics
Genes, Fungal
Genomes
Lipid Metabolism
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction - methods
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
sophorolipids
Yeast
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Summary:Three cytochrome P450 monooxygenases belonging to the CYP52 family were isolated from the genome of the sophorolipid-producing yeast Candida bombicola using degenerate PCR and genomic walking. One gene displayed high identity with the CYP52E members and was classified into this group (CYP52E3), whereas the other genes belonged to new groups: CYP52M and CYP52N. CYP52E3 and CYP52N1 turned out to be of no relevance for sophorolipid production, but show clear upregulation when the yeast cells are grown on alkanes as the sole carbon source. On the other hand, CYP52M1 is clearly upregulated during sophorolipid synthesis and very likely takes part in sophorolipid formation.
ISSN:1567-1356
1567-1364
DOI:10.1111/j.1567-1364.2008.00454.x