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Importance of the cytochrome P450 monooxygenase CYP52 family for the sophorolipid-producing yeast Candida bombicola
Three cytochrome P450 monooxygenases belonging to the CYP52 family were isolated from the genome of the sophorolipid-producing yeast Candida bombicola using degenerate PCR and genomic walking. One gene displayed high identity with the CYP52E members and was classified into this group (CYP52E3), wher...
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| Published in: | FEMS yeast research 2009-02, Vol.9 (1), p.87-94 |
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| cites | cdi_FETCH-LOGICAL-c4624-407441acfcf5402bf1ec3d08f3b54e97880ec59dac794d207582e8bb2c125f73 |
| container_end_page | 94 |
| container_issue | 1 |
| container_start_page | 87 |
| container_title | FEMS yeast research |
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| creator | Van Bogaert, Inge N.A Demey, Marjan Develter, Dirk Soetaert, Wim Vandamme, Erick J |
| description | Three cytochrome P450 monooxygenases belonging to the CYP52 family were isolated from the genome of the sophorolipid-producing yeast Candida bombicola using degenerate PCR and genomic walking. One gene displayed high identity with the CYP52E members and was classified into this group (CYP52E3), whereas the other genes belonged to new groups: CYP52M and CYP52N. CYP52E3 and CYP52N1 turned out to be of no relevance for sophorolipid production, but show clear upregulation when the yeast cells are grown on alkanes as the sole carbon source. On the other hand, CYP52M1 is clearly upregulated during sophorolipid synthesis and very likely takes part in sophorolipid formation. |
| doi_str_mv | 10.1111/j.1567-1364.2008.00454.x |
| format | article |
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One gene displayed high identity with the CYP52E members and was classified into this group (CYP52E3), whereas the other genes belonged to new groups: CYP52M and CYP52N. CYP52E3 and CYP52N1 turned out to be of no relevance for sophorolipid production, but show clear upregulation when the yeast cells are grown on alkanes as the sole carbon source. On the other hand, CYP52M1 is clearly upregulated during sophorolipid synthesis and very likely takes part in sophorolipid formation.</description><identifier>ISSN: 1567-1356</identifier><identifier>EISSN: 1567-1364</identifier><identifier>DOI: 10.1111/j.1567-1364.2008.00454.x</identifier><identifier>PMID: 19054129</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>alkane assimilation ; Alkanes ; Amino Acid Sequence ; Candida - enzymology ; Candida - genetics ; Candida - metabolism ; Candida bombicola ; Carbon sources ; CYP52 ; Cytochrome ; Cytochrome P-450 Enzyme System - genetics ; Cytochrome P-450 Enzyme System - physiology ; Cytochrome P450 ; Cytochrome P450 monooxygenase ; DNA, Fungal - genetics ; Genes, Fungal ; Genomes ; Lipid Metabolism ; Molecular Sequence Data ; Phylogeny ; Polymerase Chain Reaction - methods ; Sequence Alignment ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; sophorolipids ; Yeast</subject><ispartof>FEMS yeast research, 2009-02, Vol.9 (1), p.87-94</ispartof><rights>2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved 2008</rights><rights>2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. 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One gene displayed high identity with the CYP52E members and was classified into this group (CYP52E3), whereas the other genes belonged to new groups: CYP52M and CYP52N. CYP52E3 and CYP52N1 turned out to be of no relevance for sophorolipid production, but show clear upregulation when the yeast cells are grown on alkanes as the sole carbon source. On the other hand, CYP52M1 is clearly upregulated during sophorolipid synthesis and very likely takes part in sophorolipid formation.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>19054129</pmid><doi>10.1111/j.1567-1364.2008.00454.x</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1567-1356 |
| ispartof | FEMS yeast research, 2009-02, Vol.9 (1), p.87-94 |
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| language | eng |
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| source | OUP_牛津大学出版社OA刊 |
| subjects | alkane assimilation Alkanes Amino Acid Sequence Candida - enzymology Candida - genetics Candida - metabolism Candida bombicola Carbon sources CYP52 Cytochrome Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - physiology Cytochrome P450 Cytochrome P450 monooxygenase DNA, Fungal - genetics Genes, Fungal Genomes Lipid Metabolism Molecular Sequence Data Phylogeny Polymerase Chain Reaction - methods Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid sophorolipids Yeast |
| title | Importance of the cytochrome P450 monooxygenase CYP52 family for the sophorolipid-producing yeast Candida bombicola |
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