multispecies-based taxonomic microarray reveals interspecies hybridization and introgression in Saccharomyces cerevisiae

A multispecies-based taxonomic microarray targeting coding sequences of diverged orthologous genes in Saccharomyces cerevisiae, Saccharomyces paradoxus, Saccharomyces mikatae, Saccharomyces bayanus, Saccharomyces kudriavzevii, Naumovia castellii, Lachancea kluyveri and Candida glabrata was designed...

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Bibliographic Details
Published in:FEMS yeast research 2009-02, Vol.9 (1), p.143-152
Main Authors: Muller, Ludo A.H, McCusker, John H
Format: Article
Language:English
Subjects:
Candida glabrata - genetics
DNA microarrays
DNA, Fungal - genetics
Genome, Fungal
Genomes
Hybridization
Hybrids
interspecies hybridization
introgression
Microarray Analysis
multispecies microarray
Recombinants
Recombination, Genetic
Saccharomyces
Saccharomyces cerevisiae
Saccharomycetales - genetics
Species
Taxonomy
Yeast
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Summary:A multispecies-based taxonomic microarray targeting coding sequences of diverged orthologous genes in Saccharomyces cerevisiae, Saccharomyces paradoxus, Saccharomyces mikatae, Saccharomyces bayanus, Saccharomyces kudriavzevii, Naumovia castellii, Lachancea kluyveri and Candida glabrata was designed to allow identification of isolates of these species and their interspecies hybrids. Analysis of isolates of several Saccharomyces species and interspecies hybrids demonstrated the ability of the microarray to differentiate these yeasts on the basis of their specific hybridization patterns. Subsequent analysis of 183 supposed S. cerevisiae isolates of various ecological and geographical backgrounds revealed one misclassified S. bayanus or Saccharomyces uvarum isolate and four aneuploid interspecies hybrids, one between S. cerevisiae and S. bayanus and three between S. cerevisiae and S. kudriavzevii. Furthermore, this microarray design allowed the detection of multiple introgressed S. paradoxus DNA fragments in the genomes of three different S. cerevisiae isolates. These results show the power of multispecies-based microarrays as taxonomic tools for the identification of species and interspecies hybrids, and their ability to provide a more detailed characterization of interspecies hybrids and recombinants.
ISSN:1567-1356
1567-1364
DOI:10.1111/j.1567-1364.2008.00464.x