Lack of endosymbiont release by two Lucinidae (Bivalvia) of the genus Codakia: consequences for symbiotic relationships

• Published in: FEMS microbiology ecology 2009-02, Vol.67 (2), p.261-267
• Main Authors: Brissac, Terry, Gros, Olivier, Merçot, Hervé
• Format: Article
• Language: English
• Subjects: Adults; Animal, plant and microbial ecology; Animals; Bacteria; Bacteria - genetics; Bacteria - isolation & purification; Biological and medical sciences; Bivalvia; Bivalvia - classification; Bivalvia - growth & development; Bivalvia - microbiology; Bivalvia - ultrastructure; CARD-FISH; Chemical analysis; Chemoautotrophic bacteria; Codakia; Codakia orbicularis; Codakia orbiculata; Ecology; Fundamental and applied biological sciences. Psychology; Gills - microbiology; Gills - ultrastructure; In Situ Hybridization, Fluorescence - methods; Invertebrates; Lucinidae; Marine; Marine invertebrates; Microbial ecology; Microbiology; Microscopy, Electron, Transmission; Mollusca; Oxidation-Reduction; Seawater; Seawater - microbiology; Solemyidae; Species Specificity; Sulfur - metabolism; sulfur-oxidizing gill endosymbiont; Symbionts; Symbiosis; transmission mode; Vesicomyidae; Water analysis; Water purification
• ISSN: 0168-6496; 1574-6941
• DOI: 10.1111/j.1574-6941.2008.00626.x

Associations between marine invertebrates and chemoautotrophic bacteria constitute a wide field for the study of symbiotic associations. In these interactions, symbiont transmission must represent the cornerstone allowing the persistence of the association throughout generations. Within Bivalvia, in families such as Solemyidae or Vesicomyidae, symbiont transmission is undoubtedly vertical. However, in Lucinidae, symbiont transmission is described in the literature as 'environmental', symbionts being acquired from the environment by the new host generations. Hence, if there is transmission, symbionts should be transmitted from adults to juveniles via the environment. Consequently, we should observe a release of the symbiont by adults. We attempted to detect such a release within two Lucinidae species of the genus Codakia. We sampled 10 Codakia orbicularis and 20 Codakia orbiculata distributed in 10 crystallizing dishes containing filtered seawater. During 1 month of investigation, we analyzed water of the dishes in order to detect any release of a symbiont using catalyzed report deposition-FISH techniques. For 140 observations realized during this period, we did not observe any release of symbionts. This suggests that the idea of host-to-host passage in Lucinidae is inaccurate. We could therefore consider that the transmission mode from generation to generation does not occur within Lucinidae, symbiosis appearing to be advantageous in this case only for the host, and constitutes an evolutionary dead-end for the bacteria.