Identification and differentiation of magnetically labeled mesenchymal stem cells in vivo in swines with myocardial infarction

Abstract We aim to track mesenchymal stem cells (MSCs) after magnetically labeling and test the ability of these cells differentiate into cardiomyocytes in vivo. Therefore, 20 swines were divided into four groups, sham-operated group ( n = 3); acute myocardial infarction (AMI) transplanted with PBS...

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Bibliographic Details
Published in:International journal of cardiology 2009-01, Vol.131 (3), p.417-419
Main Authors: Qi, Chun-mei, Ma, Gen-shan, Liu, Nai-feng, Shen, Cheng-xing, Chen, Zhong, Liu, Xiao-jun, Hu, Yao-peng, Zhang, Xiao-li, Chen, Ji-yuan, Ju,Yao-liang Tang, Sheng-hong
Format: Article
Language:English
Subjects:
Animals
Arisaema
Biological and medical sciences
Cardiology. Vascular system
Cardiovascular
Cell Differentiation
Contrast media
Coronary heart disease
Differentiation
Ferrosoferric Oxide
Fluorescent Dyes
Heart
Indicators and Reagents
Magnetic Resonance Imaging
Medical sciences
Mesenchymal stem cell
Mesenchymal Stem Cell Transplantation - methods
Mesenchymal Stromal Cells - pathology
Myocardial infarction
Myocardial Infarction - pathology
Myocarditis. Cardiomyopathies
Myocytes, Cardiac - pathology
Swine
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Summary:Abstract We aim to track mesenchymal stem cells (MSCs) after magnetically labeling and test the ability of these cells differentiate into cardiomyocytes in vivo. Therefore, 20 swines were divided into four groups, sham-operated group ( n = 3); acute myocardial infarction (AMI) transplanted with PBS ( n = 3); labeled MSCs ( n = 7) and unlabeled MSCs ( n = 7) group. 107 labeled or unlabeled cells were intracoronary delivered after MI (4.8 ± 1.3 days), and serial cardiac MR (3.0T) imaging studies were performed at 0, 4 and 8 weeks after transplantation, then the results were confirmed by histological and western blot analysis. We demonstrated that labeled MSCs can be reliably detected and tracked in vivo using MR imaging. In particular, we provided the evidence of regeneration of labeled MSCs in vivo by histological examination and western blot analysis.
ISSN:0167-5273
1874-1754
DOI:10.1016/j.ijcard.2007.07.164