Cell-Laden and Cell-Free Biopolymer Hydrogel for the Treatment of Osteochondral Defects in a Sheep Model

The objective of the current study was to determine the suitability of cell-laden and cell-free alginate–gelatin biopolymer hydrogel for osteochondral restoration in a sheep model ( n  = 12). Four femoral defects per animal were filled with hydrogel (cHG) plus autologous chondrocytes (cHG + C) or pe...

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Bibliographic Details
Published in:Tissue engineering. Part A 2009-01, Vol.15 (1), p.75-82
Main Authors: Schagemann, Jan C., Erggelet, Christoph, Chung, Hsi-Wei, Lahm, Andreas, Kurz, Haymo, Mrosek, Eike H.
Format: Article
Language:English
Subjects:
Alginates - chemistry
Animals
Biocompatible Materials - chemistry
Biopolymers
Biopolymers - chemistry
Bones
Buffers
Cartilage, Articular - cytology
Cartilage, Articular - injuries
Cartilage, Articular - transplantation
Cells, Cultured
Cellular biology
Chondrocytes - transplantation
Collagen Type I - chemistry
Feasibility Studies
Fluorescent Antibody Technique, Direct
Glucuronic Acid - chemistry
Hexuronic Acids - chemistry
Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry
Hydrogen-Ion Concentration
Knee Joint - surgery
Materials Testing
Models, Animal
Periosteum - cytology
Regeneration
Sheep
Sheep, Domestic - surgery
Time Factors
Tissue engineering
Transplantation, Autologous - methods
Treatment Outcome
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Summary:The objective of the current study was to determine the suitability of cell-laden and cell-free alginate–gelatin biopolymer hydrogel for osteochondral restoration in a sheep model ( n  = 12). Four femoral defects per animal were filled with hydrogel (cHG) plus autologous chondrocytes (cHG + C) or periosteal cells (cHG + P) or gel only (cHG) or were left untreated (E). In situ solidification enabled instantaneous implant fixation. Sixteen weeks postoperatively, defect sites were processed for light microscopy and immunofluorescence. A modified Mankin and a semi-quantitative immunoreactivity score were used to evaluate histology and immunofluorescence, respectively. Defects after cHG + C were restored with smooth, hyaline-like neo-cartilage and trabecular subchondral bone. cHG + P and cHG treatments revealed slightly inferior regenerate morphology. Undifferentiated tissue was found in E. The histological score showed significant ( p  
ISSN:1937-3341
1937-335X
DOI:10.1089/ten.tea.2008.0087