Epidemiological and Serological Survey of Brucellosis in Mongolia by ELISA Using Sarcosine Extracts

Brucellosis is an important zoonosis, and serological surveillance is essential to its control. However, cross‐reactions of attenuated live cells of Brucella abortus strain S‐19 and B. melitensis strain Rev‐1 with Yersinia enterocolitica O9 or vaccinated animal sera interfere with accurate serologic...

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Bibliographic Details
Published in:Microbiology and immunology 2004-01, Vol.48 (8), p.571-577
Main Authors: Erdenebaatar, Janchivdorj, Bayarsaikhan, Balgan, Yondondorj, Agchbazar, Watarai, Masahisa, Shirahata, Toshikazu, Jargalsaikhan, Enkhtuya, Kawamoto, Keiko, Makino, Sou-ichi
Format: Article
Language:English
Subjects:
Animal Husbandry
Animals
Animals, Domestic - microbiology
Antibodies, Bacterial - blood
Antigens, Bacterial - immunology
Antigens, Bacterial - isolation & purification
Brucella abortus
Brucella abortus - immunology
Brucella melitensis
brucellosis
Brucellosis - diagnosis
Brucellosis - epidemiology
Brucellosis - veterinary
Brucellosis, Bovine - diagnosis
Brucellosis, Bovine - epidemiology
Cattle
domestic animal
elisa
Humans
mongolia
Mongolia - epidemiology
Rose Bengal
Sarcosine
Sensitivity and Specificity
seroepidemiology
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Summary:Brucellosis is an important zoonosis, and serological surveillance is essential to its control. However, cross‐reactions of attenuated live cells of Brucella abortus strain S‐19 and B. melitensis strain Rev‐1 with Yersinia enterocolitica O9 or vaccinated animal sera interfere with accurate serological diagnosis by the Rose Bengal test (RBT). Therefore, we used ELISA with sarcosine extracts from the virulent B. abortus strain 544 to eliminate false‐positives among RBT positive‐sera. A total of 697 serum samples were collected in Mongolia from humans and animals in 23 nomadic herds. The herds were classified into three groups as brucellosis‐endemic (BE), brucellosis‐suspected (BS), or Brucella‐vaccinated (BV). The number of 295 animals (43.0%) was positive by RBT, but 206 (69.8%) of these were positive according to ELISA; therefore, 30.2% of the RBT‐positive sera were found to be false positives. The false positive samples for RTB represent 4.1%, 27.4%, and 68.2% of the animals from the BE, BS, and BV herds, respectively. In addition, 32% of RBT‐positive human sera were also false positives. Thus, our ELISA would be more specific than RTB and useful for epidemiological surveillance for brucellosis.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.2004.tb03553.x