Cellular vimentin content regulates the protein level of hepatitis C virus core protein and the hepatitis C virus production in cultured cells

Abstract Hepatitis C virus (HCV) core protein is essential for virus particle formation. Using HCV core-expressing and non-expressing Huh7 cell lines, Uc39-6 and Uc321, respectively, we performed comparative proteomic studies of proteins in the 0.5% Triton X-100-insoluble fractions of cells, and fou...

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Published in:Virology (New York, N.Y.) N.Y.), 2009-01, Vol.383 (2), p.319-327
Main Authors: Nitahara-Kasahara, Yuko, Fukasawa, Masayoshi, Shinkai-Ouchi, Fumiko, Sato, Shigeko, Suzuki, Tetsuro, Murakami, Kyoko, Wakita, Takaji, Hanada, Kentaro, Miyamura, Tatsuo, Nishijima, Masahiro
Format: Article
Language:English
Subjects:
Cell Line
Core protein
Electrophoresis, Gel, Two-Dimensional
Gene Knockdown Techniques
Gene Silencing
Hepacivirus - growth & development
Hepatitis C virus
Humans
Infectious Disease
Proteome - analysis
Vimentin
Vimentin - genetics
Vimentin - metabolism
Viral Core Proteins - biosynthesis
Viral Proteins - analysis
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Summary:Abstract Hepatitis C virus (HCV) core protein is essential for virus particle formation. Using HCV core-expressing and non-expressing Huh7 cell lines, Uc39-6 and Uc321, respectively, we performed comparative proteomic studies of proteins in the 0.5% Triton X-100-insoluble fractions of cells, and found that core-expressing Uc39-6 cells had much lower vimentin content than Uc321 cells. In experiments using vimentin-overexpressing and vimentin-knocked-down cells, we demonstrated that core protein levels were affected by cellular vimentin content. When vimentin expression was knocked-down, there was no difference in mRNA level of core protein; but proteasome-dependent degradation of the core protein was strongly reduced. These findings suggest that the turnover rate of core protein is regulated by cellular vimentin content. HCV production was also affected by cellular vimentin content. Our findings together suggest that modulation of hepatic vimentin expression might enable the control of HCV production.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2008.10.009