High-performance liquid chromatography/tandem mass spectrometric assay for the simultaneous measurement of dopamine, norepinephrine, 5-hydroxytryptamine and cocaine in biological samples

A rapid liquid chromatography/tandem mass spectrometry (LC–MS/MS) method has been developed for the measurement of dopamine (DA), 5-hydroxytryptamine (5HT) and norepinephrine (NE) in brain microdialysates. The assay has also been utilised for the simultaneous measurement of these neurotransmitters a...

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Bibliographic Details
Published in:Journal of neuroscience methods 2004-09, Vol.138 (1), p.123-132
Main Authors: Hows, Mark E.P., Lacroix, Laurent, Heidbreder, Christian, Organ, Andrew J., Shah, Ajit J.
Format: Article
Language:English
Subjects:
5-Hydroxytryptamine
Animals
Biogenic Monoamines - analysis
Brain Chemistry - drug effects
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Cocaine
Cocaine - analysis
Cocaine - pharmacology
Dopamine
Extracellular Space - drug effects
Extracellular Space - metabolism
Linear Models
Male
Mass Spectrometry - instrumentation
Mass Spectrometry - methods
Microdialysis
Microdialysis - methods
Norepinephrine
Rats
Rats, Sprague-Dawley
Reproducibility of Results
RP-HPLC
Sensitivity and Specificity
Tandem mass spectrometry
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Summary:A rapid liquid chromatography/tandem mass spectrometry (LC–MS/MS) method has been developed for the measurement of dopamine (DA), 5-hydroxytryptamine (5HT) and norepinephrine (NE) in brain microdialysates. The assay has also been utilised for the simultaneous measurement of these neurotransmitters and cocaine in brain dialysates. The neurotransmitters and cocaine were resolved in a single 4-min run using a binary gradient elution profile. The analytes were detected using tandem mass spectrometry in the positive ion electrospray mode. The limits of detection for DA, NE, 5HT and cocaine were 200, 1000, 900 pM and 1 pg ml −1, respectively.
ISSN:0165-0270
1872-678X
DOI:10.1016/j.jneumeth.2004.03.021