Long-Distance Movement of Aspergillus nidulans Early Endosomes on Microtubule Tracks

In fungal hyphal cells, intracellular membrane trafficking is constrained by the relatively long intracellular distances and the mode of growth, exclusively by apical extension. Endocytosis plays a key role in hyphal tip growth, which involves the coupling of secretory membrane delivery to the apica...

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Bibliographic Details
Published in:Traffic (Copenhagen, Denmark) Denmark), 2009-01, Vol.10 (1), p.57-75
Main Authors: Abenza, Juan F, Pantazopoulou, Areti, Rodríguez, José M, Galindo, Antonio, Peñalva, Miguel A
Format: Article
Language:English
Subjects:
Aspergillus nidulans
Aspergillus nidulans - genetics
Aspergillus nidulans - metabolism
Aspergillus nidulellus
Biological Transport
Cytoplasm - metabolism
dynein
Dyneins - genetics
Dyneins - metabolism
endocytic recycling
Endosomes - metabolism
Gene Deletion
Kinesin - metabolism
Microtubules - metabolism
rab GTP-Binding Proteins - genetics
rab GTP-Binding Proteins - metabolism
Rab5
Racemases and Epimerases - genetics
Racemases and Epimerases - metabolism
synaptobrevin
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Summary:In fungal hyphal cells, intracellular membrane trafficking is constrained by the relatively long intracellular distances and the mode of growth, exclusively by apical extension. Endocytosis plays a key role in hyphal tip growth, which involves the coupling of secretory membrane delivery to the apical region with subapical compensatory endocytosis. However, the identity, dynamics and function of filamentous fungal endosomal compartments remain largely unexplored. Aspergillus nidulans RabARab⁵ localizes to a population of endosomes that show long range bidirectional movement on microtubule (MT) tracks and are labelled with FM4-64 shortly after dye internalization. RabARab⁵ membranes do not overlap with largely static mature endosomes/vacuoles. Impaired delivery of dynein to the MT plus ends or downregulation of cytoplasmic dynein using the dynein heavy chain nudA1tsmutation results in accumulation of RabARab⁵ endosomal membranes in an abnormal NudA1 compartment at the tip, strongly supporting the existence in A. nidulans hyphal tips of a dynein loading region. We show that the SynA synaptobrevin endocytic recycling cargo traffics through this region, which strongly supports the contention that polarized hyphal growth involves the association of endocytic recycling with the plus ends of MTs located at the tip, near the endocytic internalization collar.
ISSN:1398-9219
1600-0854
DOI:10.1111/j.1600-0854.2008.00848.x