DNA at Aqueous/Solid Interfaces: Chirality-Based Detection via Second Harmonic Generation Activity

We present electronic spectra of single-strand and duplex DNA oligonucleotides covalently attached to fused quartz/aqueous interfaces and demonstrate that a strong nonlinear optical linear dichroism response is obtained when adenine and thymine bases undergo Watson−Crick base pairing to form a doubl...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2009-01, Vol.131 (2), p.844-848
Main Authors: Boman, Faith C, Gibbs-Davis, Julianne M, Heckman, Laurel M, Stepp, Brian R, Nguyen, SonBinh T, Geiger, Franz M
Format: Article
Language:English
Subjects:
DNA - chemistry
DNA, Single-Stranded - chemistry
Nucleic Acid Hybridization
Oligonucleotides - chemistry
Quartz - chemistry
Stereoisomerism
Water - chemistry
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Summary:We present electronic spectra of single-strand and duplex DNA oligonucleotides covalently attached to fused quartz/aqueous interfaces and demonstrate that a strong nonlinear optical linear dichroism response is obtained when adenine and thymine bases undergo Watson−Crick base pairing to form a double helix. Complementary χ(3) charge screening studies indicate that the signal originates from 5 × 1011 strands per square centimeter, or 6 attomoles of surface-bound oligonucleotides. The label-free, molecular-specific nature afforded by nonlinear optical studies of DNA at aqueous/solid interfaces allows for the real-time tracking of interfacial DNA hybridization for the first time.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja808007b