Non-enzymatic platelet-activating factor formation by acetylated proteins

• Published in: FEBS letters 2004-08, Vol.573 (1), p.11-14
• Main Authors: Thanou, Paraskevi G., Tsoukatos, Demokritos C.
• Format: Article
• Language: English
• Subjects: Acetylated proteins; Acetylation; Animals; apo A-I, apolipoprotein A-I; Apolipoprotein A-I - chemistry; BCA, bicinchoninic acid; Blood Platelets - chemistry; BSA, bovine serum albumin; C16:0 PAF, 1-O-hexadecyl-PAF; Cations, Divalent; Chromatography, High Pressure Liquid; HDL, high-density lipoprotein; Hydrogen-Ion Concentration; Kinetics; LDL, low-density lipoprotein; Lipoproteins, LDL - chemistry; Lyso platelet-activating factor; lyso-PAF, 1-O-hexadecyl-sn-glycero-3-phosphocholine; Non-enzymatic acetylation; PAF, platelet-activating factor (1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine); PBS, phosphate buffered saline; Pefabloc, 4-[2-aminoethyl]benzenosulfonyl fluoride; Platelet Activating Factor - analogs & derivatives; Platelet Activating Factor - chemistry; Platelet Activating Factor - isolation & purification; Platelet-activating factor; Rabbits; Serum Albumin, Bovine - chemistry; Temperature
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2004.07.037

Substantial amounts of platelet-activating factor (PAF 1- O-alkyl-2-acyl-sn-glycero-3-phosphocholine), the potent phospholipid mediator of allergic and inflammatory reactions, are formed upon incubation of acetylated low-density lipoprotein, acetylated bovine serum albumin (BSA) and acetylated apolipoprotein A-I with 1-0-hexadecyl-sn-glycero-3-phosphocholine (lyso-PAF). Acetylated BSA produced 0.3 nmol PAF/mg of protein after a 6 h incubation period with 40 μM lyso-PAF. The transfer of acetate bound to acetylated proteins to lyso-PAF was non-enzymatic. Chemical PAF formation by acetylated proteins, involved in lipid metabolism and transport, could lead to complication of inflammatory and allergic events.