Triple quadrupole linear ion trap mass spectrometer for the analysis of small molecules and macromolecules

Recently, linear ion traps (LITs) have been combined with quadrupole (Q), time‐of‐flight (TOF) and Fourier transform ion cyclotron resonance (FT‐ICR) mass spectrometery (MS). LITs can be used either as ion accumulation devices or as commercially available, stand‐alone mass spectrometers with MSn cap...

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Bibliographic Details
Published in:Journal of mass spectrometry. 2004-08, Vol.39 (8), p.845-855
Main Authors: Hopfgartner, Gérard, Varesio, Emmanuel, Tschäppät, Viviane, Grivet, Chantal, Bourgogne, Emmanuel, Leuthold, Luc Alexis
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Animals
Antihypertensive Agents - analysis
Biological and medical sciences
Chemistry
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Humans
Imidazoles - analysis
linear ion trap
Mass spectrometry
metabolism
nanoelectrospray
Nanotechnology - instrumentation
Nanotechnology - methods
Organic chemistry
peptides
Peptides - analysis
Reactivity and mechanisms
Spectrometry, Mass, Electrospray Ionization - instrumentation
Spectrometry, Mass, Electrospray Ionization - methods
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Summary:Recently, linear ion traps (LITs) have been combined with quadrupole (Q), time‐of‐flight (TOF) and Fourier transform ion cyclotron resonance (FT‐ICR) mass spectrometery (MS). LITs can be used either as ion accumulation devices or as commercially available, stand‐alone mass spectrometers with MSn capabilities. The combination of triple quadrupole MS with LIT technology in the form of an instrument of configuration QqLIT, using axial ejection, is particularly interesting, because this instrument retains the classical triple quadrupole scan functions such as selected reaction monitoring (SRM), product ion (PI), neutral loss (NL) and precursor ion (PC) while also providing access to sensitive ion trap experiments. For small molecules, quantitative and qualitative analysis can be performed using the same instrument. In addition, for peptide analysis, the enhanced multiply charged (EMC) scan allows an increase in selectivity, while the time‐delayed fragmentation (TDF) scan provides additional structural information. Various methods of operating the hybrid instrument are described for the case of the commercial Q TRAP (AB/MDS Sciex) and applications to drug metabolism analysis, quantitative confirmatory analysis, peptides analysis and automated nanoelectrospray (ESI‐chip‐MS) analysis are discussed. Copyright © 2004 John Wiley & Sons, Ltd.
ISSN:1076-5174
1096-9888
DOI:10.1002/jms.659