Amperometric sensor based on ferrocene-modified multiwalled carbon nanotube nanocomposites as electron mediator for the determination of glucose

• Published in: Analytical biochemistry 2009-02, Vol.385 (2), p.264-269
• Main Authors: Qiu, Jian-Ding, Zhou, Wen-Mei, Guo, Jin, Wang, Rui, Liang, Ru-Ping
• Format: Article
• Language: English
• Subjects: Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biosensor; Chitosan; Electrodes; Enzymes, Immobilized; Ferrocenecarboxaldehyde; Ferrous Compounds - chemistry; Glucose - analysis; Glucose Oxidase; Metallocenes; Multiwalled carbon nanotubes; Nanocomposites; Nanotubes, Carbon - chemistry
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/j.ab.2008.12.002

A kind of nanocomposite with good dispersion in water was prepared through covalent adsorption of ferrocenecarboxaldehyde on multiwalled carbon nanotubes (MWNTs) for electrical communication between glucose oxidase (GOD) and electrode. The ferrocene-modified multiwalled carbon nanotube nanocomposites (MWNTs-Fc) could be conveniently cast on electrode surfaces. With the aid of chitosan, GOD was then immobilized on the nanostructure film to form a reagentless amperometric sensor for glucose determination. FTIR spectra and cyclic voltammetry were used to characterize the nanocomposites. The presence of both ferrocene as mediator of electron transfer and MWNTs as conductor enhanced greatly the enzymatic response to the oxidation of glucose. The novel biosensor exhibited a fast response toward glucose with a detection limit of 3.0 × 10 −6 mol/L and the linear range extended up to 3.8 × 10 −3 mol/L.