Differential immunological phenotypes are exhibited after scald and flame burns

A dysfunctional immune system is known to be part of the pathophysiology after burn trauma. However, reports that support this have used a variety of methods, with numerous variables, to induce thermal injury. We hypothesized that, all other parameters being equal, an injury infliction by a scald wo...

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Bibliographic Details
Published in:Shock (Augusta, Ga.) Ga.), 2009-02, Vol.31 (2), p.157-163
Main Authors: Tschöp, Johannes, Martignoni, André, Reid, Maria D, Adediran, Samuel G, Gardner, Jason, Noel, Greg J, Ogle, Cora K, Neely, Alice N, Caldwell, Charles C
Format: Article
Language:English
Subjects:
Animals
Burns - immunology
Burns - therapy
Cytokines - metabolism
Flow Cytometry
Hematocrit
Inflammation
Interferon-gamma - metabolism
Interleukin-6 - blood
Macrophages - metabolism
Male
Mice
Mice, Inbred C57BL
Phenotype
Spleen - cytology
Spleen - metabolism
T-Box Domain Proteins - biosynthesis
T-Lymphocytes - metabolism
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Summary:A dysfunctional immune system is known to be part of the pathophysiology after burn trauma. However, reports that support this have used a variety of methods, with numerous variables, to induce thermal injury. We hypothesized that, all other parameters being equal, an injury infliction by a scald would yield different immunological responses than one inflicted by a flame. Here, we demonstrated that both burn methods produced a full-thickness burn, yet there was more of an increase in subdermal temperature, hematocrit, mortality, and serum IL-6 concentrations associated with the scald burn. On postinjury day 1, the scald-burned mice showed diminished lymphocyte numbers, interferon gamma production, and lymphocyte T-bet expression as compared with sham- and flame-burned mice. On postburn day 8, spleens from both sets of thermally injured animals showed an increase in proinflammatory myeloid cells as compared with sham-burned mice. Furthermore, the T-cell numbers, T-bet expression, and phenotype were changed such that interferon gamma production was higher in scald-burned mice than in sham- and flame-burned mice. Altogether, the data show that differential immunological phenotypes were observed depending on the thermal injury method used.
ISSN:1073-2322
1540-0514
DOI:10.1097/SHK.0b013e31817fbf4d