Donor platelets stored for at least 3 days can elicit activation marker expression by the recipient's blood mononuclear cells: an in vitro study

BACKGROUND: Recent studies have demonstrated that infused platelets (PLTs) can promote inflammation. The objective of this study was to evaluate the impact of storage of transfusion‐grade PLTs on the peripheral blood mononuclear cells (PBMNCs) of the recipient. STUDY DESIGN AND METHODS: An in vitro...

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Bibliographic Details
Published in:Transfusion (Philadelphia, Pa.) Pa.), 2009-01, Vol.49 (1), p.91-98
Main Authors: Cognasse, Fabrice, Hamzeh-Cognasse, Hind, Lafarge, Sandrine, Acquart, Sophie, Chavarin, Patricia, Courbil, Rémi, Fabrigli, Patrick, Garraud, Olivier
Format: Article
Language:English
Subjects:
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Biological and medical sciences
Blood coagulation. Blood cells
Blood Platelets - cytology
Blood Platelets - metabolism
Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis
Coculture Techniques
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Humans
Inflammation - etiology
Inflammation - metabolism
Leukocytes, Mononuclear - cytology
Leukocytes, Mononuclear - metabolism
Medical sciences
Models, Biological
Molecular and cellular biology
P-Selectin - biosynthesis
Platelet
Platelet Activation
Platelet Transfusion
Preservation, Biological
Time Factors
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
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Summary:BACKGROUND: Recent studies have demonstrated that infused platelets (PLTs) can promote inflammation. The objective of this study was to evaluate the impact of storage of transfusion‐grade PLTs on the peripheral blood mononuclear cells (PBMNCs) of the recipient. STUDY DESIGN AND METHODS: An in vitro cell model system was established to measure the degree of activation of donor PLTs during 5 days of their storage and then to measure immune cell activation by detecting marker expression in coculture experiments. RESULTS: The level of soluble CD62p increased significantly by Day 3, and membrane expression of CD62p increased significantly from Day 2, indicating some degree of PLT activation over time during storage (p 
ISSN:0041-1132
1537-2995
DOI:10.1111/j.1537-2995.2008.01931.x