IGF-I mRNA levels in bovine satellite cell cultures: Effects of fusion and anabolic steroid treatment

Androgenic and estrogenic steroids enhance muscle growth in a number of species; however, the mechanism by which anabolic steroids enhance muscle growth is not known. Castrated male cattle (steers) provide a particularly good model system in which to study the effects of anabolic steroids on muscle...

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Published in:Journal of cellular physiology 2004-11, Vol.201 (2), p.181-189
Main Authors: Kamanga-Sollo, E., Pampusch, M.S., Xi, G., White, M.E., Hathaway, M.R., Dayton, W.R.
Format: Article
Language:English
Subjects:
Anabolic Agents - pharmacology
Animals
Blotting, Western
Cattle
Cell Division - drug effects
Cells, Cultured
Electrophoresis, Polyacrylamide Gel
Estradiol - pharmacology
Estrogen Receptor alpha - drug effects
Estrogen Receptor alpha - metabolism
Growth Substances - metabolism
Insulin-Like Growth Factor I - drug effects
Insulin-Like Growth Factor I - metabolism
Male
Receptors, Androgen - drug effects
Receptors, Androgen - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
RNA, Messenger - drug effects
Satellite Cells, Skeletal Muscle - drug effects
Satellite Cells, Skeletal Muscle - metabolism
Trenbolone Acetate - pharmacology
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Summary:Androgenic and estrogenic steroids enhance muscle growth in a number of species; however, the mechanism by which anabolic steroids enhance muscle growth is not known. Castrated male cattle (steers) provide a particularly good model system in which to study the effects of anabolic steroids on muscle growth because they respond dramatically to treatment with both estrogens and androgens. The goal of this study was to determine if treatment of bovine satellite cell (BSC) cultures with 17β‐estradiol (E2) or trenbolone (a synthetic androgen) directly affects proliferation rate or level of mRNA for estrogen receptor (ER)‐α, androgen receptor, and growth factors that have been shown to affect muscle growth (insulin‐like growth factor (IGF)‐I, IGF binding protein (IGFBP)‐3, and myostatin). BSC cultures were established from the semimembranosus muscles of steers and then treated for 48 h with various concentrations of E2 or trenbolone ranging from 0.001 to 10 nM. IGF‐I mRNA levels in proliferating BSC cultures were significantly increased at 0.01 (1.9‐times control values, P 
ISSN:0021-9541
1097-4652
DOI:10.1002/jcp.20000