Loading…

Determination of the purity of ampicillin by micellar electrokinetic chromatography and reversed phase liquid chromatography on a monolithic silica column

A micellar electrokinetic chromatographic (MEKC) method and a fast reversed‐phase liquid chromatographic one have been developed for determining the purity of ampicillin. MEKC separation of ampicillin and its related substances was performed with the use of an untreated fused‐silica capillary and 40...

Full description

Saved in:
Bibliographic Details
Published in:Journal of separation science 2004-05, Vol.27 (7-8), p.560-568
Main Authors: DOLEZALOVA, Milada, KUNTEOVA, Bohdana, JOBANEK, Roman
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A micellar electrokinetic chromatographic (MEKC) method and a fast reversed‐phase liquid chromatographic one have been developed for determining the purity of ampicillin. MEKC separation of ampicillin and its related substances was performed with the use of an untreated fused‐silica capillary and 40 mM phosphate‐borate buffer, pH 7.5 containing 75 mM SDS. The HPLC method employed a monolithic silica C18 column and a mobile phase composed of phosphate buffer, pH 5.2 and ACN, the flow rate being 4.0 mL/min. Both methods were successfully validated. Linearity, relative response factors, limits of quantitation, intermediate precision, and accuracy were evaluated. The methods proved to be fast, reliable, and sufficiently sensitive and, accordingly, well‐suited for control of purity of ampicillin substance, injections, and capsules. A combination of both methods can be very useful in the confirmation of impurity profiles.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200301680