The use of Cyan Fluorescent Protein variants with a distinctive lifetime signature

The use of Cyan Fluorescent Proteins, with a distinctive lifetime signature, opens up new alternatives to track and semi-quantify the relative expression of proteins in vivo using a single excitation source and emission channel.

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Bibliographic Details
Published in:Molecular bioSystems 2009-01, Vol.5 (2), p.151-153
Main Authors: Kim, Jiho, Kwon, DoYoon, Lee, Joohyun, Pasquier, Hélène, Grailhe, Regis
Format: Article
Language:English
Subjects:
Animals
Cricetinae
Directed Molecular Evolution
Genetic Techniques
Green Fluorescent Proteins - metabolism
HeLa Cells
Humans
Hydrogen-Ion Concentration
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Mutation
Protein Engineering - methods
Temperature
Time Factors
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Description
Summary:The use of Cyan Fluorescent Proteins, with a distinctive lifetime signature, opens up new alternatives to track and semi-quantify the relative expression of proteins in vivo using a single excitation source and emission channel.
ISSN:1742-206X
1742-2051
DOI:10.1039/b815445g