Endochondral ossification is required for haematopoietic stem-cell niche formation

• Published in: Nature 2009-01, Vol.457 (7228), p.490-494
• Main Authors: Weissman, Irving L, Luppen, Cynthia A, Wei, Kevin, Kraft, Daniel L, Kuo, Calvin J, Chan, Charles K. F, Helms, Jill A, Chen, Ching-Cheng, Kim, Jae-Beom, DeBoer, Anthony T
• Format: Article
• Language: English
• Subjects: Animals; Antigens, CD - metabolism; Biological and medical sciences; Bones; Cartilage - cytology; Cartilage - embryology; Cell differentiation, maturation, development, hematopoiesis; Cell physiology; Choristoma; Fetus - cytology; Fundamental and applied biological sciences. Psychology; Hematopoietic stem cells; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - metabolism; Humanities and Social Sciences; Kidneys; letter; Mandible - cytology; Mandible - embryology; Mice; Mice, Inbred C57BL; Molecular and cellular biology; multidisciplinary; Ossification; Osteogenesis - physiology; Physiological aspects; Population; Rodents; Science; Science (multidisciplinary); Skull - cytology; Skull - embryology; Sp7 Transcription Factor; Stem Cell Niche - cytology; Stem Cell Niche - physiology; Stem cells; Thy-1 Antigens - metabolism; Transcription Factors - antagonists & inhibitors; Transcription Factors - metabolism; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - antagonists & inhibitors; Vascular Endothelial Growth Factor A - metabolism
• ISSN: 0028-0836; 1476-4687; 1476-4679
• DOI: 10.1038/nature07547

Little is known about the formation of niches, local micro-environments required for stem-cell maintenance. Here we develop an in vivo assay for adult haematopoietic stem-cell (HSC) niche formation. With this assay, we identified a population of progenitor cells with surface markers CD45-Tie2- V+CD105+Thy1.1- (CD105+Thy1-) that, when sorted from 15.5 days post-coitum fetal bones and transplanted under the adult mouse kidney capsule, could recruit host-derived blood vessels, produce donor-derived ectopic bones through a cartilage intermediate and generate a marrow cavity populated by host-derived long-term reconstituting HSC (LT-HSC). In contrast, CD45-Tie2- V+CD105+Thy1+ (CD105+Thy1+) fetal bone progenitors form bone that does not contain a marrow cavity. Suppressing expression of factors involved in endochondral ossification, such as osterix and vascular endothelial growth factor (VEGF), inhibited niche generation. CD105+Thy1- progenitor populations derived from regions of the fetal mandible or calvaria that do not undergo endochondral ossification formed only bone without marrow in our assay. Collectively, our data implicate endochondral ossification, bone formation that proceeds through a cartilage intermediate, as a requirement for adult HSC niche formation.