Observation of dermal collagen fiber in wrinkled skin using polarization-resolved second-harmonic-generation microscopy

Optical probe methods for in vivo assessments of cutaneous photoaging are necessary in fields such as anti-aging dermatology and skin cosmetic development. We investigated the relation between wrinkle direction and collagen orientation in ultraviolet-B-exposed (UVB-exposed) skin using polarization-r...

Full description

Saved in:
Bibliographic Details
Published in:Optics express 2009-01, Vol.17 (2), p.912-923
Main Authors: Yasui, Takeshi, Takahashi, Yu, Fukushima, Shuichiro, Ogura, Yuki, Yamashita, Toyonobu, Kuwahara, Tomohiro, Hirao, Tetsuji, Araki, Tsutomu
Format: Article
Language:English
Subjects:
Animals
Dermis - physiology
Dermis - radiation effects
Fibrillar Collagens - metabolism
Mice
Microscopy, Polarization - methods
Skin Aging - physiology
Skin Aging - radiation effects
Ultraviolet Rays
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Optical probe methods for in vivo assessments of cutaneous photoaging are necessary in fields such as anti-aging dermatology and skin cosmetic development. We investigated the relation between wrinkle direction and collagen orientation in ultraviolet-B-exposed (UVB-exposed) skin using polarization-resolved second-harmonic-generation (SHG) microscopy. A polarization anisotropic image of the SHG light indicated that wrinkle direction in UVB-exposed skin is predominantly parallel to the orientation of dermal collagen fibers. Furthermore, collagen orientation in post-UVB-exposed skin with few wrinkles changed from that of UVB-exposed wrinkled skin to that of no-UVB-exposed skin. The method proposed has the potential to become a powerful non-invasive tool for assessment of cutaneous photoaging.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.17.000912