Adenoviral-mediated Gene transduction of the hepatocyte growth factor (HGF) antagonist, NK4, suppresses peritoneal metastases of gastric cancer in nude mice

The competitive inhibitory effects of NK4 (a specific hepatocyte growth factor (HGF)-antagonist) on the interaction between HGF and the c-Met/HGF receptor has been shown in HGF-mediated invasion of some distinct types of human cancer cells. Furthermore, NK4 has inhibitory effects on the angiogenic p...

Full description

Saved in:
Bibliographic Details
Published in:European journal of cancer (1990) 2004-09, Vol.40 (14), p.2135-2142
Main Authors: Ueda, Kentaro, Iwahashi, Makoto, Matsuura, Ichiro, Nakamori, Mikihito, Nakamura, Masaki, Ojima, Toshiyasu, Naka, Teiji, Ishida, Koichiro, Matsumoto, Kunio, Nakamura, Toshikazu, Yamaue, Hiroki
Format: Article
Language:English
Subjects:
Adenoviral vector
Adenoviridae
Animals
Antineoplastic Agents - therapeutic use
Biological and medical sciences
CDDP
Cell Line, Tumor
Cisplatin - therapeutic use
Combined Modality Therapy
Gastric cancer
Gastroenterology. Liver. Pancreas. Abdomen
Gene therapy
Hepatocyte Growth Factor - antagonists & inhibitors
HGF antagonist
Medical sciences
Mice
Mice, Nude
Microcirculation
Mitogens
NK4
Peritoneal metastasis
Peritoneal Neoplasms - blood supply
Peritoneal Neoplasms - prevention & control
Peritoneal Neoplasms - secondary
Pharmacology. Drug treatments
Stomach Neoplasms
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Transduction, Genetic - methods
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The competitive inhibitory effects of NK4 (a specific hepatocyte growth factor (HGF)-antagonist) on the interaction between HGF and the c-Met/HGF receptor has been shown in HGF-mediated invasion of some distinct types of human cancer cells. Furthermore, NK4 has inhibitory effects on the angiogenic pathways driven by basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF), as well as by HGF. In this study, to evaluate the therapeutic efficacy of adenoviral-mediated NK4 gene treatment, we employed animal models of peritoneal metastasis using two gastric cancer cell lines, the strongly c-Met expressing MKN45 cell line and the weakly c-Met-expressing cell line, TMK1. In both models, the total number and weight of peritoneal tumours per mouse and ascites treated early with AxCANK4 (administered 3 times 2, 7 and 12 days after the tumour inoculation) were significantly reduced compared with those treated with phosphate-buffered solution (PBS) and AxCALacZ ( P
ISSN:0959-8049
1879-0852
DOI:10.1016/j.ejca.2004.05.006