Prime–boost vaccination with a fowlpox vector and an inactivated avian influenza vaccine is highly immunogenic in Pekin ducks challenged with Asian H5N1 HPAI

Abstract The efficacy of different vaccination schedules was evaluated in 17-day-old Pekin ducks using an experimental inactivated whole virus vaccine based on the H5N9 A/chicken/Italy/22A/98 isolate (H5N9-It) and/or a fowlpox recombinant (vFP-H5) expressing a synthetic HA gene from an Asian H5N1 is...

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Bibliographic Details
Published in:Vaccine 2009-01, Vol.27 (5), p.646-654
Main Authors: Steensels, M, Bublot, M, Van Borm, S, De Vriese, J, Lambrecht, B, Richard-Mazet, A, Chanavat-Bizzini, S, Duboeuf, M, Le Gros, F.-X, van den Berg, T
Format: Article
Language:English
Subjects:
Age
Allergy and Immunology
Animals
Antibodies, Viral - blood
Antigens
Applied microbiology
Aquatic birds
Asian HPAI H5N1
Biological and medical sciences
Birds
Chick Embryo
Chickens
Cloaca - virology
Ducks
Female
Fowlpox
Fowlpox virus - genetics
Fundamental and applied biological sciences. Psychology
Genes
Hemagglutination Inhibition Tests
Immune system
Immunization, Secondary
Infections
Influenza A Virus, H5N1 Subtype - genetics
Influenza A Virus, H5N1 Subtype - immunology
Influenza in Birds - immunology
Influenza in Birds - prevention & control
Influenza Vaccines - genetics
Influenza Vaccines - immunology
Microbiology
Mortality
Mouth - virology
Pekin ducks
Poultry
Vaccination
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccines, Inactivated - immunology
Vaccines, Synthetic - immunology
Virus Shedding - immunology
Wildfowl
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Summary:Abstract The efficacy of different vaccination schedules was evaluated in 17-day-old Pekin ducks using an experimental inactivated whole virus vaccine based on the H5N9 A/chicken/Italy/22A/98 isolate (H5N9-It) and/or a fowlpox recombinant (vFP-H5) expressing a synthetic HA gene from an Asian H5N1 isolate (A/chicken/Indonesia/7/2003). Full protection against clinical signs and shedding was induced by the different vaccination schemes. However, the broadest antibody response and the lowest antibody increase after challenge were observed in the group of ducks whose immune system was primed with the fowlpox vectored vaccine and boosted with the inactivated vaccine, suggesting that this prime-boost strategy induced optimal immunity against H5N1 and minimal viral replication after challenge in ducks. In addition, this prime-boost vaccination scheme was shown to be immunogenic in 1-day-old ducklings.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2008.11.044