Hypertension Produced by Placental Ischemia in Pregnant Rats Is Associated With Increased Soluble Endoglin Expression

• Published in: Hypertension (Dallas, Tex. 1979) Tex. 1979), 2009-02, Vol.53 (2, Part 2 Suppl), p.399-403
• Main Authors: Gilbert, Jeffrey S, Gilbert, Sara A.B, Arany, Marietta, Granger, Joey P
• Format: Article
• Language: English
• Subjects: Animals; Arterial hypertension. Arterial hypotension; Biological and medical sciences; Blood and lymphatic vessels; Blood Pressure - physiology; Cardiology. Vascular system; Disease Models, Animal; Endoglin; Experimental diseases; Female; Heme Oxygenase-1 - metabolism; Hypertension - metabolism; Hypertension - physiopathology; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism; Intracellular Signaling Peptides and Proteins - metabolism; Ischemia - metabolism; Medical sciences; Placenta - blood supply; Pre-Eclampsia - etiology; Pre-Eclampsia - metabolism; Pregnancy; Pregnancy, Animal - metabolism; Rats; Rats, Sprague-Dawley
• ISSN: 0194-911X; 1524-4563
• DOI: 10.1161/HYPERTENSIONAHA.108.123513

Recent clinical studies indicate that an excess of angiostatic factors, such as soluble endoglin (sEng), is related to the occurrence of preeclampsia. Although recent clinical studies report that sEng is increased in preeclamptic women, the mechanisms underlying its overexpression remain unclear. Evidence suggests that hypoxia and induction of heme oxygenase-1 have opposing effects on sEng expression, the former stimulatory and the latter inhibitory. Hence, we hypothesized that placental ischemia because of reduced uterine perfusion pressure (RUPP) in the pregnant rat would increase sEng expression and decrease heme oxygenase-1. Mean arterial pressure was obtained via arterial catheter, and serum and placental proteins were measured by Western blot. Mean arterial pressure was increased (132±3 mm Hg versus 102±2 mm Hg; P