Divergent Effects of Caloric Restriction on Gene Expression in Normal and Long-Lived Mice

Long-lived Ames dwarf mice share many phenotypic characteristics with animals subjected to caloric restriction (CR) but they are not CR mimetics. CR prolongs longevity in both normal and Ames dwarf mice. Using real-time polymerase chain reaction and western blot, we have examined the expression of g...

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Bibliographic Details
Published in:The journals of gerontology. Series A, Biological sciences and medical sciences Biological sciences and medical sciences, 2004-08, Vol.59 (8), p.B784-B788
Main Authors: Masternak, Michal M., Al-Regaiey, Khalid, Bonkowski, Michael S., Panici, Jacob, Sun, Liou, Wang, Jian, Przybylski, Grzegorz K., Bartke, Andrzej
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Caloric Restriction
Female
Gene Expression - physiology
Genes
Genetics
Glucose
Insulin
Insulin - physiology
Longevity - physiology
Male
Mice
Mice, Mutant Strains
Polymerase Chain Reaction
Rodents
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Summary:Long-lived Ames dwarf mice share many phenotypic characteristics with animals subjected to caloric restriction (CR) but they are not CR mimetics. CR prolongs longevity in both normal and Ames dwarf mice. Using real-time polymerase chain reaction and western blot, we have examined the expression of genes related to insulin signaling in the liver of normal and dwarf mice subjected to 30% CR. The results revealed divergent responses of dwarf and normal animals to CR raising an interesting possibility that CR may affect longevity of normal and dwarf mice by different mechanisms. Moreover, effects of dwarfism on the expression of the examined genes differed from the effects of CR, thus adding to the evidence that these long-lived mutants are not CR mimetics.
ISSN:1079-5006
1758-535X
DOI:10.1093/gerona/59.8.B784