Optimization and validation of an enantioselective method for a chiral drug with eight stereo-isomers in capillary electrophoresis

Highly selective capillary electrophoresis (CE) screening methods were applied to find a satisfactory separation of a chiral drug with eight stereoisomeric compounds. The initial separation conditions were further optimized using response surface modelling by applying a Box‐Behnken experimental desi...

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Bibliographic Details
Published in:Electrophoresis 2004-08, Vol.25 (16), p.2876-2884
Main Authors: Jimidar, M. Ilias, Vennekens, Tom, Van Ael, Willy, Redlich, Dirk, De Smet, Maurits
Format: Article
Language:English
Subjects:
Box-Behnken experimental design
Chiral capillary electrophoresis
Chiral drug
Cyclodextrins
Electrophoresis, Capillary - methods
Electrophoresis, Capillary - statistics & numerical data
Indicators and Reagents
Molecular Structure
Pharmaceutical Preparations - chemistry
Pharmaceutical Preparations - isolation & purification
Response surface modelling
Screening methods
Solutions
Stereoisomerism
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Summary:Highly selective capillary electrophoresis (CE) screening methods were applied to find a satisfactory separation of a chiral drug with eight stereoisomeric compounds. The initial separation conditions were further optimized using response surface modelling by applying a Box‐Behnken experimental design. This approach resulted in a rapid and efficient optimization of the buffer concentration, the concentration of two cyclodextrins, and the run voltage, in order to obtain final separation conditions of the method. Further optimization and validation of the system in terms of sensitivity and robustness resulted in a method that is suitable for quality control release purposes.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.200406031