Effects of Photodynamic Therapy Evaluated in a Novel Three-dimensional Squamous Cell Carcinoma Organ Construct of the Skin

Squamous cell carcinoma (SCC) of the skin is a malignant neoplasm that occurs in all ethnic groups primarily due to chronic sun exposure and constitutes a major health problem worldwide. Novel therapies for SCC are in development but as yet no in vitro models capable of screening these therapies and...

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Published in:Photochemistry and photobiology 2009-01, Vol.85 (1), p.272-278
Main Authors: Obrigkeit, Daniela Hoeller, Jugert, Frank K., Beermann, Torsten, Baron, Jens M., Frank, Jorge, Merk, Hans F., Bickers, David R., Abuzahra, Faris
Format: Article
Language:English
Subjects:
Apoptosis
Cancer
Carcinoma, Squamous Cell - pathology
Carcinoma, Squamous Cell - radiotherapy
Cells
Cells, Cultured
Humans
Immunohistochemistry
Minority & ethnic groups
Monoclonal antibodies
Photochemotherapy
Photodynamic therapy
Radiation therapy
Skin
Skin Neoplasms - pathology
Skin Neoplasms - radiotherapy
Treatment Outcome
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Summary:Squamous cell carcinoma (SCC) of the skin is a malignant neoplasm that occurs in all ethnic groups primarily due to chronic sun exposure and constitutes a major health problem worldwide. Novel therapies for SCC are in development but as yet no in vitro models capable of screening these therapies and their mechanism of action before proceeding to clinical trials in human subjects have emerged. For this reason we have developed and characterized a novel three‐dimensional human SCC construct and validated it using photodynamic therapy (PDT), a well‐established modality for treating in situ SCCs. Histologic and immunohistochemical characterization of these SCC constructs revealed epidermal and dermal de‐differentiation, increased cell proliferation and expression of immunohistochemical markers specific for cutaneous SCC. Application of PDT to these constructs led to tumor regression with widespread apoptosis and necrosis within 5 days. This in vitro model consistently reproduces the tumor development and dynamics of growing SCCs in vivo and provides a useful approach for screening new treatment modalities for this form of cutaneous cancer.
ISSN:0031-8655
1751-1097
DOI:10.1111/j.1751-1097.2008.00432.x