Expression of senescence-associated β-galactosidase (SA-β-Gal) by human skin fibroblasts, effect of advanced glycation end-products and fucose or rhamnose-rich polysaccharides

Abstract Expression by cells of the SA-β-Gal was shown to be a reliable indicator of the switch mechanism used by cells to enter the senescent phenotype. We used this method in order to explore the variation of SA-β-Gal-positive cells with passage number and time spent in culture. Both parameters pr...

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Bibliographic Details
Published in:Archives of gerontology and geriatrics 2009-03, Vol.48 (2), p.151-154
Main Authors: Ravelojaona, V, Robert, A.M, Robert, L
Format: Article
Language:English
Subjects:
beta-Galactosidase - metabolism
Cell Line
Cell-aging
Fibroblasts - metabolism
Fucose - physiology
Glycation End Products, Advanced - physiology
Humans
Internal Medicine
Maillard reaction
Polysaccharides - physiology
Rhamnose - physiology
Rhamnose-rich oligo- and polysaccharides
Senescence-associated-β-galactosidase
Skin aging
Skin Aging - physiology
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Summary:Abstract Expression by cells of the SA-β-Gal was shown to be a reliable indicator of the switch mechanism used by cells to enter the senescent phenotype. We used this method in order to explore the variation of SA-β-Gal-positive cells with passage number and time spent in culture. Both parameters produced an increase of SA-β-Gal-positive cells. The addition of a Maillard-product (advanced glycation end-product = AGE) to the fibroblast cultures also increased SA-β-Gal expression. Fucose- and rhamnose-rich oligo- and polysaccharides (FROPs and RROPs, respectively) provided a significant protection against this AGE-induced increase of SA-β-Gal-positive cells. It is speculated that these processes might well play an important role in skin aging.
ISSN:0167-4943
1872-6976
DOI:10.1016/j.archger.2007.12.004