Prooxidative effects of TEMPO on human erythrocytes

The prooxidative effects of 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) were observed in human erythrocytes. Incubation of red blood cells with the membrane-permeable TEMPO leads to a decrease in the concentration of intracellular reduced glutathione, accompanied by the reduction of TEMPO. Extracel...

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Bibliographic Details
Published in:Cell biology international 2004-08, Vol.28 (8), p.585-591
Main Authors: Balcerczyk, Aneta, Łuczak, Katarzyna, Soszyński, Mirosław, Bartosz, Grzegorz
Format: Article
Language:English
Subjects:
Antioxidants
Cyclic N-Oxides - pharmacology
Electron paramagnetic resonance
Erythrocytes - drug effects
Erythrocytes - metabolism
Ferricyanide
Free radicals
Humans
Nitroxides
Reactive Oxygen Species - metabolism
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Summary:The prooxidative effects of 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) were observed in human erythrocytes. Incubation of red blood cells with the membrane-permeable TEMPO leads to a decrease in the concentration of intracellular reduced glutathione, accompanied by the reduction of TEMPO. Extracellular ferricyanide inhibited the loss of glutathione and reduction of TEMPO. TEMPO induced glutathione release from the cells and oxidation of hemoglobin to methemoglobin; ferricyanide prevented these effects. These results indicate that TEMPO may act as an oxidant to erythrocytes, whilst extracellular ferricyanide protects against its effects.
ISSN:1065-6995
1095-8355
DOI:10.1016/j.cellbi.2004.04.014