Electrophoretic analysis of di- and oligosaccharides derived from glycosaminoglycans on microchip format

Microchip electrophoresis is a powerful tool for fast analysis of nucleic acids and has expanded its applicability to the analysis of various biological materials including proteins and carbohydrates. Glycosaminoglycans have intrinsic negative charges, and are good targets for electrophoretic analys...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2004-09, Vol.36 (1), p.9-15
Main Authors: Matsuno, Yu-ki, Kinoshita, Mitsuhiro, Kakehi, Kazuaki
Format: Article
Language:English
Subjects:
Aminoacridines - chemistry
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Capillary electrophoresis
Chondroitin Sulfates - analysis
Disaccharides - analysis
Electrophoresis, Microchip - methods
Fluorescent Dyes
Fundamental and applied biological sciences. Psychology
General pharmacology
Glycosaminoglycan
Glycosaminoglycans - analysis
HeLa Cells
Humans
Hyaluronic Acid - analysis
Medical sciences
Microchip electrophoresis
Oligosaccharide
Oligosaccharides - analysis
Pharmacology. Drug treatments
Sensitivity and Specificity
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Summary:Microchip electrophoresis is a powerful tool for fast analysis of nucleic acids and has expanded its applicability to the analysis of various biological materials including proteins and carbohydrates. Glycosaminoglycans have intrinsic negative charges, and are good targets for electrophoretic analysis. In the present paper, we developed a method to analyze oligosaccharides and unsaturated disaccharides derived from some glycosaminoglycans after digestion with specific enzymes followed by derivatization with 2-aminoacrydone (AMAC) by reductive amination. The method described here allowed rapid analysis of oligosaccharides derived from glycosaminoglycans within 150 s with high sensitivity. We show an application of the present technique to the glycosaminoglycan analysis in cultured HeLa cells.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2004.05.020