Determination of cholesterol in food samples using dispersive liquid–liquid microextraction followed by HPLC–UV

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2009-02, Vol.877 (4), p.456-460
• Main Authors: Daneshfar, A., Khezeli, T., Lotfi, H.J.
• Format: Article
• Language: English
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Cholesterol; Cholesterol - analysis; Chromatography, High Pressure Liquid - methods; Disperser liquid–liquid microextraction; Food Analysis; Fundamental and applied biological sciences. Psychology; General pharmacology; HPLC; Hydrogen-Ion Concentration; Medical sciences; Milk; Olive oil; Pharmacology. Drug treatments; Sensitivity and Specificity; Spectrophotometry, Ultraviolet - methods; Yolk
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2008.12.050

A fast, simple, and sensitive sample preparation procedure based on dispersive liquid–liquid microextraction (DLLME) is proposed for the determination of cholesterol in food samples using isocratic reverse phase high performance liquid chromatography (RP-HPLC) and UV detection. The influence of several important parameters on extraction efficiency of cholesterol was evaluated. Under optimized conditions, a linear relationship was obtained between the peak area and the concentration of cholesterol in the range of 0.03–10 μg l −1. The detection and quantification limits were 0.01 and 0.03 μg l −1, respectively. Intra-day and inter-day precisions for the analysis of cholesterol were in the range of 1.0–3.1%. The applicability of the proposed method was demonstrated by analyzing cholesterol in milk, egg yolk and olive oil.