Surface engineering of PHBV by covalent collagen immobilization to improve cell compatibility

Covalent immobilization of collagen onto poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) (PHBV) film was achieved to improve its cell compatibility. Amide groups photografted on PHBV films were initially converted into amine groups through Hofmann degradation and collagen was then chemically bonded to...

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Bibliographic Details
Published in:Journal of Biomedical Materials Research Part B 2009-03, Vol.88A (3), p.616-627
Main Authors: Wang, Yingjun, Ke, Yu, Ren, Li, Wu, Gang, Chen, Xiaofeng, Zhao, Qichun
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Biocompatible Materials - chemistry
Biocompatible Materials - metabolism
Cell Adhesion
cell compatibility
Cells, Cultured
Chondrocytes - cytology
collagen
Collagen - chemistry
Collagen - metabolism
Collagen - ultrastructure
Male
Microscopy, Electron, Scanning
Molecular Structure
PHBV
Polyesters - chemistry
Sheep
Spectroscopy, Fourier Transform Infrared
surface modification
Surface Properties
Water - chemistry
wettability
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Summary:Covalent immobilization of collagen onto poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) (PHBV) film was achieved to improve its cell compatibility. Amide groups photografted on PHBV films were initially converted into amine groups through Hofmann degradation and collagen was then chemically bonded to amine groups, consequently forming the amide, amine, and collagen‐modified PHBV. The structures of these modified PHBV films were confirmed by ATR‐FTIR, XPS, and SEM analyses. Compared with that of PHBV film, surface wettability of the modified PHBV films enhanced remarkably. In particular, water contact angle of the collagen‐modified PHBV film decreased from 65.0° to 2.1° within 130 s. Sheep chondrocytes cultured on PHBV and modified PHBV films were evaluated by cell adhesion test, MTT assay, and morphological observation under SEM. Results showed that the collagen‐modified PHBV film had better cell adhesion and proliferation than other modified PHBV films and PHBV film. Chondrocytes on the collagen‐modified PHBV film adhered through filopodia, spread by cytoplasmic webbing, and formed cells layer earlier than other modified ones, indicating that the collagen‐modified PHBV is a promising biomaterial for cartilage tissue engineering. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009
ISSN:1549-3296
1552-4965
1552-4981
DOI:10.1002/jbm.a.31858