Reduction of molybdate by sulfate-reducing bacteria

Molybdate is an essential trace element required by biological systems including the anaerobic sulfate-reducing bacteria (SRB); however, detrimental consequences may occur if molybdate is present in high concentrations in the environment. While molybdate is a structural analog of sulfate and inhibit...

Full description

Saved in:
Bibliographic Details
Published in:Biometals 2009-02, Vol.22 (1), p.131-139
Main Authors: Biswas, Keka C, Woodards, Nicole A, Xu, Huifang, Barton, Larry L
Format: Article
Language:English
Subjects:
Bacteria
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biochemistry
Biological Transport - physiology
Biomedical and Life Sciences
Biophysics
Cell Biology
Chemical analysis
Desulfovibrio - genetics
Desulfovibrio - physiology
Desulfovibrio desulfuricans
Desulfovibrio gigas
Desulfovibrio vulgaris
Life Sciences
Medicine/Public Health
Microbiology
Molybdenum - chemistry
Molybdenum - metabolism
Oxidation-Reduction
Pharmacology/Toxicology
Plant Physiology
Seawater
Seawater - chemistry
Spectral analysis
Sulfate reduction
Sulfates
Sulfates - chemistry
Sulfates - metabolism
Sulfides
Trace elements
Water analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Molybdate is an essential trace element required by biological systems including the anaerobic sulfate-reducing bacteria (SRB); however, detrimental consequences may occur if molybdate is present in high concentrations in the environment. While molybdate is a structural analog of sulfate and inhibits sulfate respiration of SRB, little information is available concerning the effect of molybdate on pure cultures. We followed the growth of Desulfovibrio gigas ATCC 19364, Desulfovibrio vulgaris Hildenborough, Desulfovibrio desulfuricans DSM 642, and D. desulfuricans DSM 27774 in media containing sub-lethal levels of molybdate and observed a red-brown color in the culture fluid. Spectral analysis of the culture fluid revealed absorption peaks at 467, 395 and 314 nm and this color is proposed to be a molybdate-sulfide complex. Reduction of molybdate with the formation of molybdate disulfide occurs in the periplasm D. gigas and D. desulfuricans DSM 642. From these results we suggest that the occurrence of poorly crystalline Mo-sulfides in black shale may be a result from SRB reduction and selective enrichment of Mo in paleo-seawater.
ISSN:0966-0844
1572-8773
DOI:10.1007/s10534-008-9198-8