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Atrial fibrillation is associated with increased spontaneous calcium release from the sarcoplasmic reticulum in human atrial myocytes
Spontaneous Ca2+ release from the sarcoplasmic reticulum (SR) can generate afterdepolarizations, and these have the potential to initiate arrhythmias. Therefore, an association may exist between spontaneous SR Ca2+ release and initiation of atrial fibrillation (AF), but this has not yet been reporte...
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| Published in: | Circulation (New York, N.Y.) N.Y.), 2004-09, Vol.110 (11), p.1358-1363 |
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| container_end_page | 1363 |
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| container_title | Circulation (New York, N.Y.) |
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| creator | Hove-Madsen, Leif Llach, Anna Bayes-Genís, Antoni Roura, Santiago Rodriguez Font, Enrique Arís, Alejandro Cinca, Juan |
| description | Spontaneous Ca2+ release from the sarcoplasmic reticulum (SR) can generate afterdepolarizations, and these have the potential to initiate arrhythmias. Therefore, an association may exist between spontaneous SR Ca2+ release and initiation of atrial fibrillation (AF), but this has not yet been reported.
Spontaneous Ca2+ release from the SR, manifested as Ca2+ sparks and Ca2+ waves, was recorded with confocal microscopy in atrial myocytes isolated from patients with and those without AF. In addition, the spontaneous inward current associated with Ca2+ waves was measured with the use of the perforated patch-clamp technique. The Ca2+ spark frequency was higher in 8 patients with AF than in 16 patients without (6.0+/-1.2 versus 2.8+/-0.8 sparks/mm per second, P |
| doi_str_mv | 10.1161/01.CIR.0000141296.59876.87 |
| format | article |
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Spontaneous Ca2+ release from the SR, manifested as Ca2+ sparks and Ca2+ waves, was recorded with confocal microscopy in atrial myocytes isolated from patients with and those without AF. In addition, the spontaneous inward current associated with Ca2+ waves was measured with the use of the perforated patch-clamp technique. The Ca2+ spark frequency was higher in 8 patients with AF than in 16 patients without (6.0+/-1.2 versus 2.8+/-0.8 sparks/mm per second, P<0.05). Similarly, the spontaneous Ca2+ wave frequency was greater in patients with AF (2.8+/-0.5 versus 1.1+/-0.3 waves/mm per second, P<0.01). The spontaneous inward current frequency was also higher in 10 patients with AF than in 13 patients without this arrhythmia (0.101+/-0.028 versus 0.031+/-0.007 per second, P<0.05, at a clamped potential of -80 mV). In contrast, both the Ca2+ released from the SR and the Na+-Ca2+ exchange rate induced by a rapid caffeine application were comparable in patients with and without AF.
The observed increase in spontaneous Ca2+ release in patients with AF probably is due to an upregulation of the SR Ca2+ release channel activity, which may contribute to the development of AF.</description><identifier>ISSN: 0009-7322</identifier><identifier>EISSN: 1524-4539</identifier><identifier>DOI: 10.1161/01.CIR.0000141296.59876.87</identifier><identifier>PMID: 15313939</identifier><language>eng</language><publisher>United States</publisher><subject>Aged ; Atrial Appendage - physiopathology ; Atrial Appendage - ultrastructure ; Atrial Fibrillation - physiopathology ; Calcium Signaling - physiology ; Female ; Heart Atria - physiopathology ; Heart Atria - ultrastructure ; Humans ; Male ; Microscopy, Confocal ; Middle Aged ; Myocytes, Cardiac - physiology ; Myocytes, Cardiac - ultrastructure ; Patch-Clamp Techniques ; Sarcoplasmic Reticulum - secretion ; Sodium-Calcium Exchanger - metabolism</subject><ispartof>Circulation (New York, N.Y.), 2004-09, Vol.110 (11), p.1358-1363</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c531t-8c51501be1f43ba2e04c21c2bd7662764bf209ccac7b92ca142b67226e61cac63</citedby><cites>FETCH-LOGICAL-c531t-8c51501be1f43ba2e04c21c2bd7662764bf209ccac7b92ca142b67226e61cac63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15313939$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hove-Madsen, Leif</creatorcontrib><creatorcontrib>Llach, Anna</creatorcontrib><creatorcontrib>Bayes-Genís, Antoni</creatorcontrib><creatorcontrib>Roura, Santiago</creatorcontrib><creatorcontrib>Rodriguez Font, Enrique</creatorcontrib><creatorcontrib>Arís, Alejandro</creatorcontrib><creatorcontrib>Cinca, Juan</creatorcontrib><title>Atrial fibrillation is associated with increased spontaneous calcium release from the sarcoplasmic reticulum in human atrial myocytes</title><title>Circulation (New York, N.Y.)</title><addtitle>Circulation</addtitle><description>Spontaneous Ca2+ release from the sarcoplasmic reticulum (SR) can generate afterdepolarizations, and these have the potential to initiate arrhythmias. Therefore, an association may exist between spontaneous SR Ca2+ release and initiation of atrial fibrillation (AF), but this has not yet been reported.
Spontaneous Ca2+ release from the SR, manifested as Ca2+ sparks and Ca2+ waves, was recorded with confocal microscopy in atrial myocytes isolated from patients with and those without AF. In addition, the spontaneous inward current associated with Ca2+ waves was measured with the use of the perforated patch-clamp technique. The Ca2+ spark frequency was higher in 8 patients with AF than in 16 patients without (6.0+/-1.2 versus 2.8+/-0.8 sparks/mm per second, P<0.05). Similarly, the spontaneous Ca2+ wave frequency was greater in patients with AF (2.8+/-0.5 versus 1.1+/-0.3 waves/mm per second, P<0.01). The spontaneous inward current frequency was also higher in 10 patients with AF than in 13 patients without this arrhythmia (0.101+/-0.028 versus 0.031+/-0.007 per second, P<0.05, at a clamped potential of -80 mV). In contrast, both the Ca2+ released from the SR and the Na+-Ca2+ exchange rate induced by a rapid caffeine application were comparable in patients with and without AF.
The observed increase in spontaneous Ca2+ release in patients with AF probably is due to an upregulation of the SR Ca2+ release channel activity, which may contribute to the development of AF.</description><subject>Aged</subject><subject>Atrial Appendage - physiopathology</subject><subject>Atrial Appendage - ultrastructure</subject><subject>Atrial Fibrillation - physiopathology</subject><subject>Calcium Signaling - physiology</subject><subject>Female</subject><subject>Heart Atria - physiopathology</subject><subject>Heart Atria - ultrastructure</subject><subject>Humans</subject><subject>Male</subject><subject>Microscopy, Confocal</subject><subject>Middle Aged</subject><subject>Myocytes, Cardiac - physiology</subject><subject>Myocytes, Cardiac - ultrastructure</subject><subject>Patch-Clamp Techniques</subject><subject>Sarcoplasmic Reticulum - secretion</subject><subject>Sodium-Calcium Exchanger - metabolism</subject><issn>0009-7322</issn><issn>1524-4539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqFkc2KFDEQgIMo7rj6ChI8iJduU_nteFsGdRcWBNFzSNekmUj_jEkamQfwvc3sDOzNzSVU6quqFB8h74C1ABo-Mmi3d99bVg9I4Fa3ynZGt515RjaguGykEvY52VTANkZwfkVe5fyrhloY9ZJcgRIgrLAb8vempOhHOsQ-xXH0JS4zjZn6nBeMvoQd_RPLnsYZU_C5hvmwzMXPYVkzRT9iXCeawnhK0iEtEy37QLNPuBxGn6eINVsirmPl4kz36-Rn6s9Tp-OCxxLya_Ji8GMOby73Nfn55fOP7W1z_-3r3fbmvsH64dJ0qEAx6AMMUvSeByaRA_J-Z7TmRst-4MwiejS95ehB8l4bznXQUB-1uCbvz30Pafm9hlzcFDOGuvfDPk7rTlstT-CH_4LQGdUpI614sicYbRm3XQU_nUFMS84pDO6Q4uTT0QFzJ7GOgati3aNY9yDWdaYWv71MWfsp7B5LLybFP7yVois</recordid><startdate>20040914</startdate><enddate>20040914</enddate><creator>Hove-Madsen, Leif</creator><creator>Llach, Anna</creator><creator>Bayes-Genís, Antoni</creator><creator>Roura, Santiago</creator><creator>Rodriguez Font, Enrique</creator><creator>Arís, Alejandro</creator><creator>Cinca, Juan</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>20040914</creationdate><title>Atrial fibrillation is associated with increased spontaneous calcium release from the sarcoplasmic reticulum in human atrial myocytes</title><author>Hove-Madsen, Leif ; Llach, Anna ; Bayes-Genís, Antoni ; Roura, Santiago ; Rodriguez Font, Enrique ; Arís, Alejandro ; Cinca, Juan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c531t-8c51501be1f43ba2e04c21c2bd7662764bf209ccac7b92ca142b67226e61cac63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Aged</topic><topic>Atrial Appendage - physiopathology</topic><topic>Atrial Appendage - ultrastructure</topic><topic>Atrial Fibrillation - physiopathology</topic><topic>Calcium Signaling - physiology</topic><topic>Female</topic><topic>Heart Atria - physiopathology</topic><topic>Heart Atria - ultrastructure</topic><topic>Humans</topic><topic>Male</topic><topic>Microscopy, Confocal</topic><topic>Middle Aged</topic><topic>Myocytes, Cardiac - physiology</topic><topic>Myocytes, Cardiac - ultrastructure</topic><topic>Patch-Clamp Techniques</topic><topic>Sarcoplasmic Reticulum - secretion</topic><topic>Sodium-Calcium Exchanger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hove-Madsen, Leif</creatorcontrib><creatorcontrib>Llach, Anna</creatorcontrib><creatorcontrib>Bayes-Genís, Antoni</creatorcontrib><creatorcontrib>Roura, Santiago</creatorcontrib><creatorcontrib>Rodriguez Font, Enrique</creatorcontrib><creatorcontrib>Arís, Alejandro</creatorcontrib><creatorcontrib>Cinca, Juan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Circulation (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hove-Madsen, Leif</au><au>Llach, Anna</au><au>Bayes-Genís, Antoni</au><au>Roura, Santiago</au><au>Rodriguez Font, Enrique</au><au>Arís, Alejandro</au><au>Cinca, Juan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Atrial fibrillation is associated with increased spontaneous calcium release from the sarcoplasmic reticulum in human atrial myocytes</atitle><jtitle>Circulation (New York, N.Y.)</jtitle><addtitle>Circulation</addtitle><date>2004-09-14</date><risdate>2004</risdate><volume>110</volume><issue>11</issue><spage>1358</spage><epage>1363</epage><pages>1358-1363</pages><issn>0009-7322</issn><eissn>1524-4539</eissn><abstract>Spontaneous Ca2+ release from the sarcoplasmic reticulum (SR) can generate afterdepolarizations, and these have the potential to initiate arrhythmias. Therefore, an association may exist between spontaneous SR Ca2+ release and initiation of atrial fibrillation (AF), but this has not yet been reported.
Spontaneous Ca2+ release from the SR, manifested as Ca2+ sparks and Ca2+ waves, was recorded with confocal microscopy in atrial myocytes isolated from patients with and those without AF. In addition, the spontaneous inward current associated with Ca2+ waves was measured with the use of the perforated patch-clamp technique. The Ca2+ spark frequency was higher in 8 patients with AF than in 16 patients without (6.0+/-1.2 versus 2.8+/-0.8 sparks/mm per second, P<0.05). Similarly, the spontaneous Ca2+ wave frequency was greater in patients with AF (2.8+/-0.5 versus 1.1+/-0.3 waves/mm per second, P<0.01). The spontaneous inward current frequency was also higher in 10 patients with AF than in 13 patients without this arrhythmia (0.101+/-0.028 versus 0.031+/-0.007 per second, P<0.05, at a clamped potential of -80 mV). In contrast, both the Ca2+ released from the SR and the Na+-Ca2+ exchange rate induced by a rapid caffeine application were comparable in patients with and without AF.
The observed increase in spontaneous Ca2+ release in patients with AF probably is due to an upregulation of the SR Ca2+ release channel activity, which may contribute to the development of AF.</abstract><cop>United States</cop><pmid>15313939</pmid><doi>10.1161/01.CIR.0000141296.59876.87</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aged Atrial Appendage - physiopathology Atrial Appendage - ultrastructure Atrial Fibrillation - physiopathology Calcium Signaling - physiology Female Heart Atria - physiopathology Heart Atria - ultrastructure Humans Male Microscopy, Confocal Middle Aged Myocytes, Cardiac - physiology Myocytes, Cardiac - ultrastructure Patch-Clamp Techniques Sarcoplasmic Reticulum - secretion Sodium-Calcium Exchanger - metabolism |
| title | Atrial fibrillation is associated with increased spontaneous calcium release from the sarcoplasmic reticulum in human atrial myocytes |
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