Expression of the LIM-homeodomain gene Lmx1a in the postnatal mouse central nervous system

Abstract The LIM-homeodomain transcription factor Lmx1a plays critical roles in roof plate formation as well as in the cell fate determination of midbrain dopaminergic neurons during embryonic development, but its function in the adult brain remains unknown. In the present study, as the first step i...

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Bibliographic Details
Published in:Brain research bulletin 2009-03, Vol.78 (6), p.306-312
Main Authors: Zou, Hui-Li, Su, Chang-Jun, Shi, Ming, Zhao, Guang-Yu, Li, Zhu-Yi, Guo, Chao, Ding, Yu-Qiang
Format: Article
Language:English
Subjects:
Animals
Brain
Brain - growth & development
Brain - metabolism
Cell Count
Distribution
Dopamine
Dreher
Gene Expression
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Immunohistochemistry
In Situ Hybridization
LIM-Homeodomain Proteins
Lmx1a
Lmx1b
Mice
Mice, Inbred C57BL
Neurology
RNA, Messenger - genetics
RNA, Messenger - metabolism
Spinal Cord - growth & development
Spinal Cord - metabolism
Transcription Factors
Tyrosine 3-Monooxygenase - metabolism
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Summary:Abstract The LIM-homeodomain transcription factor Lmx1a plays critical roles in roof plate formation as well as in the cell fate determination of midbrain dopaminergic neurons during embryonic development, but its function in the adult brain remains unknown. In the present study, as the first step in exploring its function in adult brain, we examined the expression of Lmx1a in the mouse central nervous system (CNS) from birth to adulthood by in situ hybridization. Lmx1a was expressed at high levels in the posterior hypothalamic area, supremammillary nucleus, ventral premammillary nucleus, subthalamic nucleus, ventral tegmental area, compact part of the substantia nigra and parabrachial nucleus from birth to adulthood, and co-localized with its paralogue Lmx1b in these regions. On the other hand, Lmx1a expression in the cochlear nuclei, medial cerebellar nucleus and superior vestibular nucleus was only observed until postnatal day (P) 30 and showed no colocalization with Lmx1b. Lmx1a -expressing neurons in the ventral midbrain were dopaminergic as evidenced by co-expression with tyrosine hydroxylase in these regions. Furthermore, Lmx1a expression was also found in the choroid plexuses and ependymal cells, although its expression was only detected during the first two postnatal weeks. These results suggest that Lmx1a may be involved in postnatal development as well as in maintenance of some aspects of normal brain function.
ISSN:0361-9230
1873-2747
DOI:10.1016/j.brainresbull.2008.12.001